Detailed information NTG0550688

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: J5X95_RS02065
• Genome accession: NZ_CP072120
• Coordinates: 405436..406050 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain GKT04
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 400436..411050

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J5X95_RS02035 (J5X95_02035)	rpsT	400739..401005 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
J5X95_RS02040 (J5X95_02040)	holA	401022..402063 (-)	1042	Protein_406	DNA polymerase III subunit delta	-
J5X95_RS02045 (J5X95_02045)	-	402103..402254 (-)	152	Protein_407	hypothetical protein	-
J5X95_RS02050 (J5X95_02050)	-	402295..402429 (+)	135	WP_003152870.1	YqzM family protein	-
J5X95_RS02055 (J5X95_02055)	comEC	402448..404799 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
J5X95_RS02060 (J5X95_02060)	-	404800..405369 (-)	570	WP_094031848.1	ComE operon protein 2	-
J5X95_RS02065 (J5X95_02065)	comEA	405436..406050 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
J5X95_RS02070 (J5X95_02070)	comER	406109..406930 (+)	822	WP_012118027.1	late competence protein ComER	-
J5X95_RS02075 (J5X95_02075)	-	406999..407736 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
J5X95_RS02080 (J5X95_02080)	rsfS	407733..408089 (-)	357	WP_007408260.1	ribosome silencing factor	-
J5X95_RS02085 (J5X95_02085)	yqeK	408107..408667 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
J5X95_RS02090 (J5X95_02090)	-	408657..409226 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
J5X95_RS02095 (J5X95_02095)	yhbY	409237..409527 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
J5X95_RS02100 (J5X95_02100)	aroE	409521..410363 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=550688 J5X95_RS02065 WP_043021637.1 405436..406050(-) (comEA) [Bacillus amyloliquefaciens strain GKT04]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=550688 J5X95_RS02065 WP_043021637.1 405436..406050(-) (comEA) [Bacillus amyloliquefaciens strain GKT04]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363