Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CAL6303_RS08725 Genome accession   NC_019751
Coordinates   2147041..2149053 (+) Length   670 a.a.
NCBI ID   WP_015197479.1    Uniprot ID   K9V097
Organism   Calothrix sp. PCC 6303     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2142041..2154053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAL6303_RS08715 (Cal6303_1793) dnaK 2143764..2145722 (-) 1959 WP_015197477.1 molecular chaperone DnaK -
  CAL6303_RS08720 (Cal6303_1794) grpE 2145976..2146623 (-) 648 WP_015197478.1 nucleotide exchange factor GrpE -
  CAL6303_RS08725 (Cal6303_1795) pilB/pilB1 2147041..2149053 (+) 2013 WP_015197479.1 GspE/PulE family protein Machinery gene
  CAL6303_RS08730 (Cal6303_1796) pilT 2149098..2150216 (+) 1119 WP_015197480.1 type IV pilus twitching motility protein PilT Machinery gene
  CAL6303_RS08735 (Cal6303_1797) - 2150366..2151586 (+) 1221 WP_015197481.1 type II secretion system F family protein -
  CAL6303_RS08740 (Cal6303_1798) - 2151693..2152532 (-) 840 WP_015197482.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74973.59 Da        Isoelectric Point: 4.9566

>NTDB_id=54939 CAL6303_RS08725 WP_015197479.1 2147041..2149053(+) (pilB/pilB1) [Calothrix sp. PCC 6303]
MTYSSQQRRSTALATRAEFSPFGNKLVQVGFVDTEQMRQALIESRKSGRPLTEVLESISGRQLSPELLRLYKKQQLFELK
ILYGVEYLDPEVSPVGDSQVGNLIETLIPVDICRRHRLLPLSRNDAQNPPSVLVAMVDPDNLEASDDLNRILRPQNLALQ
RMVITQEDYQQIINQYLDELAVKQKHLEKEKFTDINQDLENLADLDLAEAPDDMDVDLGSAMKGAEDAPIISLVNRILAK
ALSEKVSDIHIEPQEENLRIRFRRDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRVFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLGIVQDMVSRPFGLILVTGPTGSGKTTSLYSALAERNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDRETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGVEPFMVSSSLLGVLAQRLVRRVCTDCRIAYNPSTEELARYGLSASQDVDLTFYKANSLTNEQIQQAKANNSLCPTCGG
VGYKGRCGVYEVMRVTERLQTLINEEAPTERIKEVAVEDGMKTLLSYSLDLVRQGQTTLEEVERVTYTDTGLEAELKARR
KSSLTCRTCSAELKPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=54939 CAL6303_RS08725 WP_015197479.1 2147041..2149053(+) (pilB/pilB1) [Calothrix sp. PCC 6303]
ATGACTTACTCGTCACAACAACGACGCAGCACTGCCCTAGCTACCAGAGCAGAATTTTCTCCCTTTGGCAATAAGCTAGT
ACAAGTTGGCTTTGTCGATACTGAACAAATGCGGCAAGCGCTAATCGAAAGCCGCAAGTCTGGAAGACCCCTAACTGAAG
TATTGGAGTCAATTTCTGGACGACAACTCTCACCTGAATTGCTCAGACTTTATAAGAAGCAGCAGCTGTTTGAACTAAAA
ATACTCTACGGTGTGGAATATCTCGATCCGGAAGTTAGTCCGGTGGGAGATTCACAAGTGGGAAATTTGATTGAGACTCT
CATCCCAGTAGATATTTGTCGTCGTCATCGTTTATTGCCTTTGTCGCGTAATGATGCTCAAAATCCACCATCGGTACTTG
TGGCAATGGTGGATCCTGATAATTTGGAAGCATCAGATGACCTCAACCGAATTTTGCGACCGCAAAATTTAGCTTTGCAA
CGGATGGTAATTACTCAAGAAGATTACCAACAAATCATCAATCAATATTTAGATGAACTTGCGGTTAAACAAAAGCATTT
AGAGAAAGAAAAATTTACAGATATTAACCAGGACTTGGAAAATCTTGCCGATCTTGATTTGGCAGAGGCTCCAGATGATA
TGGATGTTGATTTAGGTTCAGCAATGAAAGGGGCTGAAGATGCTCCAATTATTAGCTTGGTGAACCGAATTTTAGCTAAA
GCCTTATCTGAGAAAGTTTCGGATATTCATATTGAACCGCAAGAAGAGAATCTTCGCATCCGCTTTCGTCGAGATGGTGT
GTTGCGAGAGGCTTTTGACCCATTACCCAAAAAAATTATTCCTGCGGTTACGGCTCGATTTAAAATCATCTCCAATTTAG
ATATTGCTGAACGACGTTTACCCCAAGATGGTCGCATCCGCCGGGTTTTTGAAGGACGCAAGGTGGATTTCCGGGTAAAT
ACACTACCTAGCCGCTACGGCGAGAAGGTTGTGTTACGGATTTTGGATAACTCCTCAACTCAATTAGGGTTAGATAAATT
AATTACTGACCCGGAAACTTTGGGGATTGTTCAGGATATGGTCAGTCGTCCCTTTGGATTGATTTTGGTGACAGGACCGA
CTGGTTCAGGTAAAACCACATCGCTATATTCGGCATTAGCGGAACGTAATGACCCTGGAATCAATATTAGTACTGTAGAG
GATCCAATTGAGTACAGTTTACCGGGGATTACTCAGGTACAGGTAATAAGGGAAAAAGGGCTAGATTTTGCTACGGCATT
ACGGGCATTCTTACGCCAAGATCCGGATGTGCTACTGGTGGGGGAAACGCGAGATCGGGAAACCGCAAAAACTGCCATCG
AAGCGGCATTAACCGGACACTTAGTTTTAACAACGCTACATACCAATGATGCACCAGGTGCGATCGCACGTTTGGGTGAA
ATGGGTGTTGAACCATTTATGGTCTCTAGCTCCCTACTTGGTGTTTTGGCACAACGTCTTGTTCGTCGTGTCTGTACAGA
TTGTCGTATTGCTTACAATCCTTCTACTGAAGAATTGGCACGCTATGGTCTATCTGCTTCTCAAGATGTAGATCTGACTT
TTTATAAAGCAAATTCCCTCACTAACGAGCAAATTCAACAGGCTAAGGCAAATAATTCACTTTGCCCAACTTGCGGGGGT
GTTGGTTACAAAGGACGCTGCGGTGTTTATGAGGTGATGCGGGTAACTGAACGTCTGCAAACTCTAATTAATGAGGAAGC
ACCCACCGAACGCATCAAGGAAGTTGCGGTTGAAGATGGGATGAAAACTCTACTTTCTTACAGCCTAGATTTGGTACGTC
AAGGACAAACAACTTTGGAAGAAGTTGAGCGGGTGACCTATACAGATACTGGTTTAGAAGCAGAACTGAAGGCAAGACGT
AAAAGTAGCCTCACTTGTCGAACCTGTAGTGCAGAACTCAAACCAGAATGGCTGGATTGTCCTTACTGTATGACACCGCG
TTTTCAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9V097

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.172

100

0.666

Multiple sequence alignment