Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PLE7327_RS20510 Genome accession   NC_019689
Coordinates   4556074..4557177 (-) Length   367 a.a.
NCBI ID   WP_015145682.1    Uniprot ID   K9TBL2
Organism   Pleurocapsa sp. PCC 7327     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4551074..4562177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PLE7327_RS25470 - 4551111..4551317 (+) 207 WP_144266154.1 hypothetical protein -
  PLE7327_RS20490 (Ple7327_4261) - 4551362..4551700 (+) 339 WP_015145678.1 hypothetical protein -
  PLE7327_RS20495 (Ple7327_4262) - 4551813..4554362 (-) 2550 WP_015145679.1 secretin N-terminal domain-containing protein -
  PLE7327_RS20500 (Ple7327_4263) - 4554463..4555233 (-) 771 WP_015145680.1 hypothetical protein -
  PLE7327_RS20505 (Ple7327_4264) - 4555230..4556069 (-) 840 WP_015145681.1 PilN domain-containing protein -
  PLE7327_RS20510 (Ple7327_4265) pilM 4556074..4557177 (-) 1104 WP_015145682.1 type IV pilus assembly protein PilM Machinery gene
  PLE7327_RS20515 (Ple7327_4266) - 4557357..4558631 (+) 1275 WP_015145683.1 sugar ABC transporter substrate-binding protein -
  PLE7327_RS25020 - 4558663..4558836 (+) 174 WP_186005345.1 hypothetical protein -
  PLE7327_RS20520 (Ple7327_4267) dxs 4559041..4560951 (+) 1911 WP_015145684.1 1-deoxy-D-xylulose-5-phosphate synthase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40920.14 Da        Isoelectric Point: 4.4269

>NTDB_id=54769 PLE7327_RS20510 WP_015145682.1 4556074..4557177(-) (pilM) [Pleurocapsa sp. PCC 7327]
MLERLKSLLPTKSTGFGLEITPERINLAQLSKQGQTYKLVKYCSSEIPEGVFEEGKIVDSLTLAERIQELLKEYKIKAKR
VATAVPMREAIIRLLPLPAELDEQELRDLVLNHEASLYLPFPREEVDLDYQKLGYFEDEDGIEKVQVLLVATRREVTDSY
IETFKQAGLEVDVLEINSFALIRTIREQLRQFGSKEAAVLVDIEFDSTEIAIVVDGVPQFSRTVPIGTFQMQTALARAMN
LPPSRDPEILRDITIPNTPFDSLSTQGIAVNPGMSALLRIVSELTDELRRSINFYLNQSEGLEVVQLLLAGPGGAIGQLD
EFFTQRLSVPTMQIDPVSALSLQIDREIPAIARPGLGTVLGLGLREV

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=54769 PLE7327_RS20510 WP_015145682.1 4556074..4557177(-) (pilM) [Pleurocapsa sp. PCC 7327]
ATGCTGGAGCGTTTAAAAAGTCTTCTACCAACCAAAAGTACGGGGTTTGGGTTAGAAATCACTCCTGAGAGGATTAACTT
AGCACAACTAAGCAAACAAGGGCAAACGTACAAACTCGTTAAATATTGTTCTTCGGAAATCCCCGAAGGCGTATTTGAAG
AGGGCAAAATTGTTGACTCGCTAACCTTAGCCGAACGCATTCAAGAACTGCTCAAAGAATACAAAATTAAAGCAAAGCGG
GTAGCAACGGCAGTGCCGATGCGAGAAGCGATTATTCGCCTTCTTCCCCTACCAGCAGAACTAGACGAGCAAGAATTGCG
GGATTTGGTTTTGAACCACGAAGCGAGTTTATATTTGCCTTTTCCCCGCGAGGAAGTGGATCTCGACTATCAAAAGTTAG
GCTATTTTGAAGACGAAGATGGCATCGAAAAAGTCCAAGTCCTATTGGTAGCCACCCGGCGAGAGGTGACTGACTCTTAT
ATAGAGACTTTCAAACAAGCTGGATTGGAAGTAGATGTCTTAGAAATCAATAGCTTTGCCTTAATTCGCACGATTAGGGA
GCAACTGAGGCAATTTGGCTCGAAAGAAGCTGCCGTCCTCGTCGATATCGAATTCGACAGTACGGAAATTGCCATTGTCG
TCGATGGGGTTCCTCAATTTTCTCGAACAGTGCCCATTGGAACATTTCAAATGCAGACGGCTTTGGCTCGCGCCATGAAT
TTACCGCCGTCGAGAGATCCTGAAATTCTCAGAGATATTACCATTCCCAACACGCCCTTCGACAGTCTGAGCACTCAAGG
GATAGCCGTAAATCCGGGGATGAGCGCTTTGCTGAGAATTGTCAGCGAACTGACAGACGAACTGCGTCGCTCGATTAATT
TCTATTTAAATCAGAGCGAGGGATTGGAAGTCGTTCAACTCCTCCTGGCAGGTCCCGGCGGCGCTATCGGACAACTAGAT
GAATTTTTTACGCAGCGCTTGAGCGTTCCGACCATGCAAATCGATCCAGTGTCAGCACTATCATTACAAATCGATCGCGA
AATTCCTGCAATCGCGCGACCGGGATTGGGAACTGTTCTGGGTTTAGGACTTCGAGAGGTTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9TBL2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

77.049

99.728

0.768

Multiple sequence alignment