Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LIO20_RS26200 Genome accession   NZ_CP085082
Coordinates   5678256..5678879 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PAO1-Holloway     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5673256..5683879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIO20_RS26180 (LIO20_26140) aroB 5673851..5674957 (-) 1107 WP_003114574.1 3-dehydroquinate synthase -
  LIO20_RS26185 (LIO20_26145) aroK 5675007..5675525 (-) 519 WP_003095833.1 shikimate kinase AroK -
  LIO20_RS26190 (LIO20_26150) pilQ 5675537..5677681 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  LIO20_RS26195 (LIO20_26155) comL/pilP 5677735..5678259 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  LIO20_RS26200 (LIO20_26160) pilO 5678256..5678879 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  LIO20_RS26205 (LIO20_26165) pilN 5678876..5679472 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  LIO20_RS26210 (LIO20_26170) pilM 5679472..5680536 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  LIO20_RS26215 (LIO20_26175) - 5680721..5683189 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=537045 LIO20_RS26200 WP_003103268.1 5678256..5678879(-) (pilO) [Pseudomonas aeruginosa strain PAO1-Holloway]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=537045 LIO20_RS26200 WP_003103268.1 5678256..5678879(-) (pilO) [Pseudomonas aeruginosa strain PAO1-Holloway]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401