Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JNO42_RS20100 Genome accession   NZ_CP069080
Coordinates   4358913..4360175 (-) Length   420 a.a.
NCBI ID   WP_143494596.1    Uniprot ID   -
Organism   Pseudomonas putida strain LWPZF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4353913..4365175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO42_RS20085 (JNO42_19960) - 4355579..4357180 (-) 1602 WP_012053948.1 AAA family ATPase -
  JNO42_RS20090 (JNO42_19965) aroB 4357196..4358293 (-) 1098 WP_003249149.1 3-dehydroquinate synthase -
  JNO42_RS20095 (JNO42_19970) aroK 4358390..4358908 (-) 519 WP_010955625.1 shikimate kinase AroK -
  JNO42_RS20100 (JNO42_19975) pilQ 4358913..4360175 (-) 1263 WP_143494596.1 secretin N-terminal domain-containing protein Machinery gene
  JNO42_RS20105 (JNO42_19980) - 4360099..4361100 (-) 1002 WP_143494673.1 pilus assembly protein PilP -
  JNO42_RS20110 (JNO42_19985) - 4361112..4361648 (-) 537 WP_239606896.1 PilN domain-containing protein -
  JNO42_RS20115 (JNO42_19990) pilM 4361645..4362526 (-) 882 WP_143494593.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45660.33 Da        Isoelectric Point: 4.9839

>NTDB_id=532214 JNO42_RS20100 WP_143494596.1 4358913..4360175(-) (pilQ) [Pseudomonas putida strain LWPZF]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGGISTGSLSVDERT
NTLVAYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=532214 JNO42_RS20100 WP_143494596.1 4358913..4360175(-) (pilQ) [Pseudomonas putida strain LWPZF]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGCAGCGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGACGCGCAGCTGCAGCCATTGCGTCGGGAACTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCGGCATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGCGTACCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGTTGGTGGCGCAGCTGGACGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAACTGTTCGTCGACCTCGGTCTGGAGCGAGCAGGCAGCAGTATCGGCCTG
GGTCTGTTGCGCGGTGACGTGTTGCTGGATCTGGAACTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACTCCGGACAACAAGGTG
ATCATGACCGTCCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.633

100

0.619
  comQ Acinetobacter baylyi ADP1

42.889

100

0.46
  pilQ Acinetobacter baumannii D1279779

40.998

100

0.45
  pilQ Acinetobacter baumannii strain A118

40.998

100

0.45
  pilQ Legionella pneumophila strain Lp02

43.269

99.048

0.429
  pilQ Legionella pneumophila strain ERS1305867

43.269

99.048

0.429
  pilQ Vibrio campbellii strain DS40M4

41.014

100

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

98.81

0.419
  pilQ Vibrio cholerae strain A1552

42.41

98.81

0.419