Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JEQ16_RS11875 Genome accession   NZ_CP066377
Coordinates   2452123..2453193 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain ID-A01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2447123..2458193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JEQ16_RS11825 (JEQ16_11765) - 2447183..2447767 (-) 585 WP_060562614.1 signal peptidase I -
  JEQ16_RS11830 (JEQ16_11770) tapA 2447739..2448410 (-) 672 WP_278071167.1 amyloid fiber anchoring/assembly protein TapA -
  JEQ16_RS11835 (JEQ16_11775) - 2448669..2448998 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  JEQ16_RS11840 (JEQ16_11780) - 2449038..2449217 (-) 180 WP_003153093.1 YqzE family protein -
  JEQ16_RS11845 (JEQ16_11785) comGG 2449274..2449651 (-) 378 WP_063174754.1 competence type IV pilus minor pilin ComGG Machinery gene
  JEQ16_RS11850 (JEQ16_11790) comGF 2449652..2450047 (-) 396 WP_094247733.1 competence type IV pilus minor pilin ComGF -
  JEQ16_RS11855 (JEQ16_11795) comGE 2450061..2450375 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  JEQ16_RS11860 (JEQ16_11800) comGD 2450359..2450796 (-) 438 WP_278071168.1 competence type IV pilus minor pilin ComGD Machinery gene
  JEQ16_RS11865 (JEQ16_11805) comGC 2450786..2451094 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  JEQ16_RS11870 (JEQ16_11810) comGB 2451099..2452136 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  JEQ16_RS11875 (JEQ16_11815) comGA 2452123..2453193 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  JEQ16_RS11880 (JEQ16_11820) - 2453385..2454335 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  JEQ16_RS11885 (JEQ16_11825) - 2454481..2455782 (+) 1302 WP_070082114.1 hemolysin family protein -
  JEQ16_RS11890 (JEQ16_11830) - 2455820..2456650 (-) 831 WP_070082115.1 STAS domain-containing protein -
  JEQ16_RS11900 (JEQ16_11840) - 2456856..2457236 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JEQ16_RS11905 (JEQ16_11845) - 2457473..2457718 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=519600 JEQ16_RS11875 WP_003153083.1 2452123..2453193(-) (comGA) [Bacillus velezensis strain ID-A01]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=519600 JEQ16_RS11875 WP_003153083.1 2452123..2453193(-) (comGA) [Bacillus velezensis strain ID-A01]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAATGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374