Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JFU10_RS12300 Genome accession   NZ_CP066337
Coordinates   2507491..2507928 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Mr12     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2502491..2512928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFU10_RS20165 - 2503089..2503217 (-) 129 Protein_2403 hypothetical protein -
  JFU10_RS12270 - 2503362..2504564 (+) 1203 WP_070081342.1 GTP-binding protein -
  JFU10_RS12275 - 2504629..2505765 (+) 1137 WP_198863482.1 zinc-dependent alcohol dehydrogenase -
  JFU10_RS12280 - 2505780..2506214 (+) 435 WP_021494203.1 RDD family protein -
  JFU10_RS12285 - 2506332..2506607 (+) 276 WP_369719109.1 YckD family protein -
  JFU10_RS12290 - 2506821..2507051 (+) 231 Protein_2408 family 1 glycosylhydrolase -
  JFU10_RS12295 nin/comJ 2507070..2507468 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  JFU10_RS12300 nucA/comI 2507491..2507928 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  JFU10_RS12305 hxlB 2508287..2508844 (-) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  JFU10_RS12310 hxlA 2508841..2509476 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  JFU10_RS12315 - 2509708..2510070 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=519335 JFU10_RS12300 WP_003156588.1 2507491..2507928(-) (nucA/comI) [Bacillus velezensis strain Mr12]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=519335 JFU10_RS12300 WP_003156588.1 2507491..2507928(-) (nucA/comI) [Bacillus velezensis strain Mr12]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641