Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I8N73_RS12925 Genome accession   NZ_CP065791
Coordinates   2665516..2666586 (-) Length   356 a.a.
NCBI ID   WP_043020785.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBUM279     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2660516..2671586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N73_RS12875 (I8N73_12875) sipW 2660576..2661160 (-) 585 WP_199022043.1 signal peptidase I SipW -
  I8N73_RS12880 (I8N73_12880) tapA 2661132..2661803 (-) 672 WP_064115390.1 amyloid fiber anchoring/assembly protein TapA -
  I8N73_RS12885 (I8N73_12885) - 2662062..2662391 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  I8N73_RS12890 (I8N73_12890) - 2662431..2662610 (-) 180 WP_003153093.1 YqzE family protein -
  I8N73_RS12895 (I8N73_12895) comGG 2662667..2663044 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  I8N73_RS12900 (I8N73_12900) comGF 2663045..2663440 (-) 396 WP_064115389.1 competence type IV pilus minor pilin ComGF -
  I8N73_RS12905 (I8N73_12905) comGE 2663454..2663768 (-) 315 WP_064115388.1 competence type IV pilus minor pilin ComGE -
  I8N73_RS12910 (I8N73_12910) comGD 2663752..2664189 (-) 438 WP_094032243.1 competence type IV pilus minor pilin ComGD Machinery gene
  I8N73_RS12915 (I8N73_12915) comGC 2664179..2664487 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  I8N73_RS12920 (I8N73_12920) comGB 2664492..2665529 (-) 1038 WP_199022044.1 competence type IV pilus assembly protein ComGB Machinery gene
  I8N73_RS12925 (I8N73_12925) comGA 2665516..2666586 (-) 1071 WP_043020785.1 competence type IV pilus ATPase ComGA Machinery gene
  I8N73_RS12930 (I8N73_12930) - 2666783..2667733 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  I8N73_RS12935 (I8N73_12935) - 2667879..2669180 (+) 1302 WP_012117986.1 hemolysin family protein -
  I8N73_RS12940 (I8N73_12940) - 2669218..2670048 (-) 831 WP_007408317.1 STAS domain-containing protein -
  I8N73_RS12950 (I8N73_12950) - 2670255..2670635 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  I8N73_RS12955 (I8N73_12955) - 2670873..2671118 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=514555 I8N73_RS12925 WP_043020785.1 2665516..2666586(-) (comGA) [Bacillus velezensis strain LBUM279]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTQRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=514555 I8N73_RS12925 WP_043020785.1 2665516..2666586(-) (comGA) [Bacillus velezensis strain LBUM279]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGGCACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACAACGTGCAGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379