Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6G73_RS21710 Genome accession   NZ_CP065651
Coordinates   4687721..4689427 (+) Length   568 a.a.
NCBI ID   WP_045525681.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain FDAARGOS_916     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4682721..4694427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G73_RS21685 (I6G73_21685) pdhR 4683870..4684634 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  I6G73_RS21690 (I6G73_21690) ampD 4684910..4685482 (-) 573 WP_045525676.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6G73_RS21695 (I6G73_21695) - 4685630..4686100 (+) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  I6G73_RS21700 (I6G73_21700) nadC 4686106..4686963 (+) 858 WP_045525678.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6G73_RS21705 (I6G73_21705) - 4687286..4687717 (+) 432 WP_106551646.1 pilin -
  I6G73_RS21710 (I6G73_21710) pilB 4687721..4689427 (+) 1707 WP_045525681.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  I6G73_RS21715 (I6G73_21715) pilC 4689543..4690784 (+) 1242 WP_045525683.1 type II secretion system F family protein Machinery gene
  I6G73_RS21720 (I6G73_21720) pilD 4690807..4691679 (+) 873 WP_045525685.1 prepilin peptidase Machinery gene
  I6G73_RS21725 (I6G73_21725) coaE 4691699..4692313 (+) 615 WP_045525686.1 dephospho-CoA kinase -
  I6G73_RS21730 (I6G73_21730) zapD 4692351..4693073 (+) 723 WP_024945431.1 cell division protein ZapD -
  I6G73_RS21735 (I6G73_21735) yacG 4693083..4693277 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  I6G73_RS21740 (I6G73_21740) mutT 4693338..4693751 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62497.87 Da        Isoelectric Point: 5.4342

>NTDB_id=512644 I6G73_RS21710 WP_045525681.1 4687721..4689427(+) (pilB) [Aeromonas hydrophila strain FDAARGOS_916]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=512644 I6G73_RS21710 WP_045525681.1 4687721..4689427(+) (pilB) [Aeromonas hydrophila strain FDAARGOS_916]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
CCTGAGTCAAGCCAAGGCCCAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTAGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTCTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTAGAAGAGAACA
AACTCACCACTGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAATTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGACAGAAGGCGCAATATCTGCGTGCTCTGTCGAAACCTCAG
GGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCTGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGTGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACTCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTAGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAACTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTGGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCTGGCCTTGAGAAGGCTCGCCTGGGTGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373