Detailed information
Bioinformatically predicted
Overview
| Name | amiF | Type | Regulator |
| Locus tag | STMM20_RS03115 | Genome accession | NZ_CP065485 |
| Coordinates | 591979..592908 (+) | Length | 309 a.a. |
| NCBI ID | WP_002951426.1 | Uniprot ID | A0A7U7CIU5 |
| Organism | Streptococcus thermophilus strain MM20 | ||
| Function | internalize XIP (predicted from homology) Competence regulation |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 541963..608439 | 591979..592908 | within | 0 |
Gene organization within MGE regions
Location: 541963..608439
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| STMM20_RS02865 (STMM20_02860) | - | 542060..542800 (-) | 741 | WP_024703961.1 | amino acid ABC transporter ATP-binding protein | - |
| STMM20_RS02870 (STMM20_02865) | - | 542800..545010 (-) | 2211 | WP_011226350.1 | ABC transporter substrate-binding protein/permease | - |
| STMM20_RS02875 (STMM20_02870) | - | 545208..546145 (+) | 938 | Protein_527 | type II CAAX endopeptidase family protein | - |
| STMM20_RS02880 (STMM20_02875) | - | 546218..546685 (+) | 468 | WP_014608580.1 | hypothetical protein | - |
| STMM20_RS02885 (STMM20_02880) | uvrB | 546832..548823 (+) | 1992 | WP_014608579.1 | excinuclease ABC subunit UvrB | - |
| STMM20_RS02890 (STMM20_02885) | - | 548868..549332 (+) | 465 | WP_011681454.1 | 8-oxo-dGTP diphosphatase | - |
| STMM20_RS02895 (STMM20_02890) | - | 549546..550376 (+) | 831 | WP_014621829.1 | transporter substrate-binding domain-containing protein | - |
| STMM20_RS02900 (STMM20_02895) | - | 550373..551170 (+) | 798 | WP_002951495.1 | transporter substrate-binding domain-containing protein | - |
| STMM20_RS02905 (STMM20_02900) | - | 551517..552626 (+) | 1110 | WP_014727642.1 | aminotransferase | - |
| STMM20_RS02910 (STMM20_02905) | - | 552688..553482 (+) | 795 | WP_023909836.1 | ABC transporter substrate-binding protein | - |
| STMM20_RS02915 (STMM20_02910) | rpsU | 553677..553853 (+) | 177 | WP_220016117.1 | 30S ribosomal protein S21 | - |
| STMM20_RS02920 (STMM20_02915) | mscL | 554013..554096 (-) | 84 | Protein_536 | large conductance mechanosensitive channel protein MscL | - |
| STMM20_RS02925 (STMM20_02920) | dnaG | 554236..556047 (+) | 1812 | WP_220016116.1 | DNA primase | - |
| STMM20_RS02930 (STMM20_02925) | rpoD | 556051..557160 (+) | 1110 | WP_011227440.1 | RNA polymerase sigma factor RpoD | - |
| STMM20_RS02935 (STMM20_02930) | - | 557214..557549 (+) | 336 | WP_002891474.1 | metal-sulfur cluster assembly factor | - |
| STMM20_RS02940 (STMM20_02935) | - | 557883..559298 (+) | 1416 | WP_014727640.1 | DUF2142 domain-containing protein | - |
| STMM20_RS02945 (STMM20_02940) | - | 559358..560278 (+) | 921 | WP_116920321.1 | glycosyltransferase family 2 protein | - |
| STMM20_RS02950 | - | 560391..560996 (+) | 606 | WP_347103615.1 | DUF6056 family protein | - |
| STMM20_RS02955 | - | 560927..561724 (+) | 798 | WP_347103616.1 | DUF6056 family protein | - |
| STMM20_RS02960 (STMM20_02950) | rfbD | 561801..562652 (+) | 852 | WP_100284792.1 | dTDP-4-dehydrorhamnose reductase | - |
| STMM20_RS02965 (STMM20_02955) | - | 562729..563424 (+) | 696 | WP_024703954.1 | glycosyltransferase family 2 protein | - |
| STMM20_RS02970 (STMM20_02960) | - | 563430..563762 (+) | 333 | WP_084829000.1 | DUF2304 domain-containing protein | - |
| STMM20_RS02975 (STMM20_02965) | - | 563755..565029 (+) | 1275 | WP_347103617.1 | lipopolysaccharide biosynthesis protein | - |
| STMM20_RS02980 (STMM20_02970) | - | 565029..566045 (+) | 1017 | WP_347103618.1 | glycosyltransferase | - |
| STMM20_RS02985 (STMM20_02975) | - | 566154..567302 (+) | 1149 | WP_347103619.1 | glycosyltransferase family 1 protein | - |
| STMM20_RS02990 (STMM20_02980) | - | 567299..568255 (+) | 957 | WP_014727634.1 | glycosyltransferase family 2 protein | - |
| STMM20_RS02995 (STMM20_02985) | - | 568239..569048 (+) | 810 | WP_002948020.1 | ABC transporter permease | - |
| STMM20_RS03000 (STMM20_02990) | - | 569048..570253 (+) | 1206 | WP_014727633.1 | ABC transporter ATP-binding protein | - |
| STMM20_RS03005 (STMM20_02995) | - | 570273..571328 (+) | 1056 | WP_002948022.1 | glycosyltransferase | - |
| STMM20_RS03010 (STMM20_03000) | - | 571325..573109 (+) | 1785 | WP_014727631.1 | rhamnan synthesis F family protein | - |
| STMM20_RS03015 (STMM20_03005) | radC | 573158..573844 (+) | 687 | WP_164135544.1 | DNA repair protein RadC | - |
| STMM20_RS03020 (STMM20_03010) | - | 573897..574592 (-) | 696 | WP_014608557.1 | gamma-glutamyl-gamma-aminobutyrate hydrolase family protein | - |
| STMM20_RS03025 (STMM20_03015) | - | 574585..575244 (-) | 660 | WP_071417293.1 | redox-sensing transcriptional repressor Rex | - |
| STMM20_RS03030 (STMM20_03020) | - | 575388..575735 (-) | 348 | WP_014608555.1 | DUF1831 domain-containing protein | - |
| STMM20_RS03035 (STMM20_03025) | - | 575737..576849 (-) | 1113 | WP_014608554.1 | cysteine desulfurase family protein | - |
| STMM20_RS03040 (STMM20_03030) | - | 576853..577824 (-) | 972 | WP_002951446.1 | ribose-phosphate diphosphokinase | - |
| STMM20_RS03045 (STMM20_03035) | - | 578187..578762 (-) | 576 | WP_002951444.1 | CYTH domain-containing protein | - |
| STMM20_RS03050 (STMM20_03040) | - | 578858..579532 (+) | 675 | WP_011681427.1 | GTP pyrophosphokinase family protein | - |
| STMM20_RS03055 (STMM20_03045) | - | 579504..580340 (+) | 837 | WP_014608552.1 | NAD kinase | - |
| STMM20_RS03060 (STMM20_03050) | - | 580337..581233 (+) | 897 | WP_014727627.1 | RluA family pseudouridine synthase | - |
| STMM20_RS03065 (STMM20_03055) | pta | 581247..582230 (+) | 984 | WP_011227420.1 | phosphate acetyltransferase | - |
| STMM20_RS03070 (STMM20_03060) | - | 582355..583669 (+) | 1315 | Protein_566 | IS3 family transposase | - |
| STMM20_RS03075 (STMM20_03065) | - | 583715..584920 (-) | 1206 | WP_347103620.1 | OFA family MFS transporter | - |
| STMM20_RS03080 (STMM20_03070) | - | 585267..585627 (+) | 361 | Protein_568 | TatD family hydrolase | - |
| STMM20_RS03085 | - | 585622..586023 (+) | 402 | Protein_569 | ABC transporter ATP-binding protein | - |
| STMM20_RS03090 (STMM20_03085) | - | 586017..586207 (+) | 191 | Protein_570 | IS3 family transposase | - |
| STMM20_RS03095 (STMM20_03090) | amiA3 | 586441..588411 (+) | 1971 | WP_347103621.1 | peptide ABC transporter substrate-binding protein | Regulator |
| STMM20_RS03100 (STMM20_03095) | amiC | 588472..589965 (+) | 1494 | WP_064355564.1 | ABC transporter permease | Regulator |
| STMM20_RS03105 (STMM20_03100) | amiD | 589965..590891 (+) | 927 | WP_084828982.1 | oligopeptide ABC transporter permease OppC | Regulator |
| STMM20_RS03110 (STMM20_03105) | amiE | 590901..591986 (+) | 1086 | WP_084828981.1 | ABC transporter ATP-binding protein | Regulator |
| STMM20_RS03115 (STMM20_03110) | amiF | 591979..592908 (+) | 930 | WP_002951426.1 | ATP-binding cassette domain-containing protein | Regulator |
| STMM20_RS03120 (STMM20_03115) | - | 592958..593743 (-) | 786 | WP_347103622.1 | ABC transporter permease | - |
| STMM20_RS03125 (STMM20_03120) | - | 593755..594453 (-) | 699 | WP_113870321.1 | ABC transporter ATP-binding protein | - |
| STMM20_RS03130 (STMM20_03125) | - | 594458..594835 (-) | 378 | WP_011681413.1 | GntR family transcriptional regulator | - |
| STMM20_RS03135 (STMM20_03130) | - | 595020..595814 (+) | 795 | WP_011681412.1 | HAD family hydrolase | - |
| STMM20_RS03140 (STMM20_03135) | - | 595807..596622 (+) | 816 | WP_024010001.1 | Cof-type HAD-IIB family hydrolase | - |
| STMM20_RS03145 (STMM20_03140) | ftsY | 596636..598027 (+) | 1392 | WP_014608538.1 | signal recognition particle-docking protein FtsY | - |
| STMM20_RS03150 (STMM20_03145) | gmk | 598253..598882 (+) | 630 | WP_014608537.1 | guanylate kinase | - |
| STMM20_RS03155 (STMM20_03150) | rpoZ | 598904..599218 (+) | 315 | WP_002951415.1 | DNA-directed RNA polymerase subunit omega | - |
| STMM20_RS03160 (STMM20_03155) | - | 599360..601756 (+) | 2397 | WP_014608535.1 | primosomal protein N' | - |
| STMM20_RS03165 (STMM20_03160) | fmt | 601774..602709 (+) | 936 | WP_014608534.1 | methionyl-tRNA formyltransferase | - |
| STMM20_RS03170 (STMM20_03165) | rsmB | 602699..604021 (+) | 1323 | WP_347103623.1 | 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB | - |
| STMM20_RS03175 (STMM20_03170) | - | 604065..604802 (+) | 738 | WP_014727615.1 | Stp1/IreP family PP2C-type Ser/Thr phosphatase | - |
| STMM20_RS03180 (STMM20_03175) | stkP/pknB | 604802..606673 (+) | 1872 | WP_347103624.1 | Stk1 family PASTA domain-containing Ser/Thr kinase | Regulator |
| STMM20_RS03185 | - | 607311..607478 (+) | 168 | WP_014608530.1 | potassium channel family protein | - |
| STMM20_RS03190 (STMM20_03185) | liaF | 607733..608431 (+) | 699 | WP_014621793.1 | cell wall-active antibiotics response protein LiaF | - |
Sequence
Protein
Download Length: 309 a.a. Molecular weight: 35171.28 Da Isoelectric Point: 6.5989
>NTDB_id=510477 STMM20_RS03115 WP_002951426.1 591979..592908(+) (amiF) [Streptococcus thermophilus strain MM20]
MPEKLVEVKDVEISFGEGRKKFVAVHNANFFINKGETFSLVGESGSGKTTIGRAIIGLNDTSNGEIIFDGKKINGYLSHS
EKNDLIRRIQMIFQDPAASLNERATVDYILSEGLYNFHLYKDEEERKAKIKEIIKEVGLLEEHLTRYPHEFSGGQRQRIG
IARSLVMQPDLVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFISDRIAVIYKGTIVEVAETEELYN
NPIHPYTKSLLSAVPIPDPILERKKVLKVYDPNQHDYSVDKPEMVEVRPGHFVWGNKTEIETYRKEQSK
MPEKLVEVKDVEISFGEGRKKFVAVHNANFFINKGETFSLVGESGSGKTTIGRAIIGLNDTSNGEIIFDGKKINGYLSHS
EKNDLIRRIQMIFQDPAASLNERATVDYILSEGLYNFHLYKDEEERKAKIKEIIKEVGLLEEHLTRYPHEFSGGQRQRIG
IARSLVMQPDLVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFISDRIAVIYKGTIVEVAETEELYN
NPIHPYTKSLLSAVPIPDPILERKKVLKVYDPNQHDYSVDKPEMVEVRPGHFVWGNKTEIETYRKEQSK
Nucleotide
Download Length: 930 bp
>NTDB_id=510477 STMM20_RS03115 WP_002951426.1 591979..592908(+) (amiF) [Streptococcus thermophilus strain MM20]
ATGCCTGAGAAATTAGTTGAAGTAAAAGATGTGGAAATTTCCTTCGGCGAAGGAAGAAAGAAGTTCGTTGCTGTCCACAA
TGCTAATTTTTTCATCAACAAGGGTGAAACCTTCTCCCTCGTTGGTGAGTCTGGTAGTGGTAAAACGACTATTGGACGTG
CCATTATCGGTTTAAATGACACAAGTAATGGTGAGATTATTTTTGACGGTAAGAAGATCAATGGATACTTATCTCACTCT
GAGAAAAACGACCTTATCCGTCGTATTCAGATGATTTTCCAAGACCCTGCGGCTAGTTTGAATGAACGTGCGACAGTCGA
TTATATCTTGTCTGAGGGCTTGTACAATTTCCATCTTTATAAAGATGAGGAAGAACGTAAGGCTAAAATCAAGGAAATCA
TCAAAGAAGTAGGACTTCTTGAGGAGCACTTAACACGTTACCCTCACGAATTTTCTGGGGGACAACGTCAACGTATCGGG
ATTGCGCGTTCTTTGGTCATGCAGCCTGATTTGGTTATCGCTGATGAACCAATCTCAGCCCTTGACGTGTCAGTTCGTGC
CCAAGTTTTGAATTTGCTTAAGAAATTCCAAAAAGAGTTGGGGTTAACCTATCTCTTTATCGCTCACGATTTGTCAGTGG
TCCGTTTCATTTCTGATCGTATCGCTGTTATCTATAAGGGGACAATCGTGGAAGTTGCTGAGACAGAAGAGCTCTACAAC
AATCCTATCCATCCTTACACCAAGTCACTCTTGTCTGCTGTTCCTATTCCAGATCCAATCTTGGAACGTAAGAAAGTCTT
GAAGGTTTATGATCCAAACCAACACGACTATTCGGTTGATAAACCAGAAATGGTGGAAGTACGCCCAGGTCACTTCGTTT
GGGGTAACAAGACAGAAATTGAGACTTATCGTAAAGAACAAAGTAAGTAA
ATGCCTGAGAAATTAGTTGAAGTAAAAGATGTGGAAATTTCCTTCGGCGAAGGAAGAAAGAAGTTCGTTGCTGTCCACAA
TGCTAATTTTTTCATCAACAAGGGTGAAACCTTCTCCCTCGTTGGTGAGTCTGGTAGTGGTAAAACGACTATTGGACGTG
CCATTATCGGTTTAAATGACACAAGTAATGGTGAGATTATTTTTGACGGTAAGAAGATCAATGGATACTTATCTCACTCT
GAGAAAAACGACCTTATCCGTCGTATTCAGATGATTTTCCAAGACCCTGCGGCTAGTTTGAATGAACGTGCGACAGTCGA
TTATATCTTGTCTGAGGGCTTGTACAATTTCCATCTTTATAAAGATGAGGAAGAACGTAAGGCTAAAATCAAGGAAATCA
TCAAAGAAGTAGGACTTCTTGAGGAGCACTTAACACGTTACCCTCACGAATTTTCTGGGGGACAACGTCAACGTATCGGG
ATTGCGCGTTCTTTGGTCATGCAGCCTGATTTGGTTATCGCTGATGAACCAATCTCAGCCCTTGACGTGTCAGTTCGTGC
CCAAGTTTTGAATTTGCTTAAGAAATTCCAAAAAGAGTTGGGGTTAACCTATCTCTTTATCGCTCACGATTTGTCAGTGG
TCCGTTTCATTTCTGATCGTATCGCTGTTATCTATAAGGGGACAATCGTGGAAGTTGCTGAGACAGAAGAGCTCTACAAC
AATCCTATCCATCCTTACACCAAGTCACTCTTGTCTGCTGTTCCTATTCCAGATCCAATCTTGGAACGTAAGAAAGTCTT
GAAGGTTTATGATCCAAACCAACACGACTATTCGGTTGATAAACCAGAAATGGTGGAAGTACGCCCAGGTCACTTCGTTT
GGGGTAACAAGACAGAAATTGAGACTTATCGTAAAGAACAAAGTAAGTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| amiF | Streptococcus thermophilus LMG 18311 |
100 |
100 |
1 |
| amiF | Streptococcus thermophilus LMD-9 |
99.676 |
100 |
0.997 |
| amiF | Streptococcus salivarius strain HSISS4 |
98.058 |
100 |
0.981 |