Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   D7S_RS08830 Genome accession   NC_017846
Coordinates   1768078..1768629 (-) Length   183 a.a.
NCBI ID   WP_005551171.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans D7S-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1763078..1773629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7S_RS08805 (D7S_01885) - 1763335..1763787 (-) 453 WP_005539334.1 methyltransferase family protein -
  D7S_RS08810 (D7S_01886) - 1763840..1764454 (-) 615 WP_005539337.1 YfgM family protein -
  D7S_RS08815 (D7S_01887) hisS 1764465..1765742 (-) 1278 WP_005551169.1 histidine--tRNA ligase -
  D7S_RS08820 (D7S_01888) ispG 1765764..1766867 (-) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  D7S_RS08825 (D7S_01889) - 1766879..1767934 (-) 1056 WP_005542473.1 helix-turn-helix domain-containing protein -
  D7S_RS08830 (D7S_01890) pilF/pilF2 1768078..1768629 (-) 552 WP_005551171.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  D7S_RS08835 (D7S_01891) - 1768651..1769811 (-) 1161 WP_005594622.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  D7S_RS08840 (D7S_01893) ansB 1770085..1771134 (+) 1050 WP_005542467.1 L-asparaginase 2 -
  D7S_RS08845 (D7S_01894) - 1771236..1772600 (-) 1365 WP_005542466.1 L-serine ammonia-lyase -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20594.34 Da        Isoelectric Point: 8.0841

>NTDB_id=50975 D7S_RS08830 WP_005551171.1 1768078..1768629(-) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans D7S-1]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKACSYAPDYYLVSAAFAYF
YQQQGDIEQARAAYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPERAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=50975 D7S_RS08830 WP_005551171.1 1768078..1768629(-) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans D7S-1]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAACAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTGTTCTTACGCTCCAGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTGCCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTCCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTACGAAAACTTGGCGCTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCCCCGGAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

56.97

90.164

0.514
  pilF Glaesserella parasuis strain SC1401

47.059

92.896

0.437

Multiple sequence alignment