Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   HWI27_RS00195 Genome accession   NZ_CP064587
Coordinates   44493..46121 (-) Length   542 a.a.
NCBI ID   WP_001186604.1    Uniprot ID   Q8VUV3
Organism   Staphylococcus epidermidis strain UMCG354     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 37728..53154 44493..46121 within 0


Gene organization within MGE regions


Location: 37728..53154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI27_RS00160 (HWI27_00160) mecA 37728..39737 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  HWI27_RS00165 (HWI27_00165) - 39834..40820 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  HWI27_RS00170 (HWI27_00170) - 40810..41052 (+) 243 Protein_31 type I restriction enzyme endonuclease domain-containing protein -
  HWI27_RS00175 (HWI27_00175) - 41043..42653 (-) 1611 Protein_32 IS1182 family transposase -
  HWI27_RS00180 (HWI27_00180) - 42702..43208 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  HWI27_RS00185 (HWI27_00185) - 43223..43534 (-) 312 WP_000700851.1 DUF960 family protein -
  HWI27_RS00190 (HWI27_00190) - 43621..43971 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  HWI27_RS00195 (HWI27_00195) ccrB 44493..46121 (-) 1629 WP_001186604.1 cassette chromosome recombinase CcrB Machinery gene
  HWI27_RS00200 (HWI27_00200) ccrA 46143..47492 (-) 1350 WP_000815652.1 cassette chromosome recombinase CcrA Machinery gene
  HWI27_RS00205 (HWI27_00205) cch1 47726..49513 (-) 1788 WP_000122621.1 cassette chromosome replicative helicase -
  HWI27_RS00210 (HWI27_00210) ssb 49513..49803 (-) 291 WP_001036437.1 cassette chromosome ssDNA-binding protein -
  HWI27_RS00215 (HWI27_00215) - 49942..50991 (+) 1050 WP_000613248.1 hypothetical protein -
  HWI27_RS00220 (HWI27_00220) - 51664..53154 (+) 1491 WP_000554504.1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62650.85 Da        Isoelectric Point: 9.9805

>NTDB_id=503290 HWI27_RS00195 WP_001186604.1 44493..46121(-) (ccrB) [Staphylococcus epidermidis strain UMCG354]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
IYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVARVNQENKVDGAALHHDIAYKQQQYD
EVQTKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKSLFDAKQISKLLQHIFQDIKHMEKSRLK
ALYLSVIDRINIRKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=503290 HWI27_RS00195 WP_001186604.1 44493..46121(-) (ccrB) [Staphylococcus epidermidis strain UMCG354]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGATCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATAGATTGTGTTATG
ATCTACAAAACAAACCGATTAGCTCGTAATACATCGGATCTTCTGAAAATCGTCGAAGATTTGCATAAACAAAATGTCGA
ATTTTTCAGTTTATCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGGCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATAGCCAATGCATTGAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTTCATGGTAAAGGAACGA
ATCTGCTTACAGGCATTATCCATTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACAACGAATACACTTAAAGAT
GGGACCAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTAATGAAGCAAATACTCGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTTG
TAGCACGTGTTAATCAAGAAAATAAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAACCAAACTAAATAACTTGATTAAAACCATAGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGTCTT
TATTTGATGCCAAACAAATCAGTAAACTATTACAACACATCTTTCAAGATATTAAGCACATGGAAAAATCTCGACTCAAA
GCATTGTACCTATCAGTGATTGATCGTATTAATATTAGAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VUV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

97.417

100

0.974