Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NRG857_RS16650 Genome accession   NC_017634
Coordinates   3449765..3450889 (-) Length   374 a.a.
NCBI ID   WP_000228517.1    Uniprot ID   E2QFE2
Organism   Escherichia coli O83:H1 str. NRG 857C     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3444765..3455889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG857_RS16620 yrdA 3446565..3447119 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  NRG857_RS16625 (NRG857_16250) yrdB 3447095..3447352 (-) 258 WP_001070558.1 DUF1488 domain-containing protein -
  NRG857_RS16630 (NRG857_16255) aroE 3447349..3448167 (-) 819 WP_000451241.1 shikimate dehydrogenase -
  NRG857_RS16635 (NRG857_16260) tsaC 3448172..3448744 (-) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  NRG857_RS16640 (NRG857_16265) yrdD 3448749..3449291 (-) 543 WP_014640218.1 type I DNA topoisomerase -
  NRG857_RS16645 (NRG857_16270) smg 3449320..3449793 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  NRG857_RS16650 (NRG857_16275) dprA 3449765..3450889 (-) 1125 WP_000228517.1 DNA-protecting protein DprA Machinery gene
  NRG857_RS16655 (NRG857_16280) def 3451019..3451528 (+) 510 WP_000114984.1 peptide deformylase -
  NRG857_RS16660 (NRG857_16285) fmt 3451543..3452490 (+) 948 WP_000004421.1 methionyl-tRNA formyltransferase -
  NRG857_RS16665 (NRG857_16290) rsmB 3452542..3453831 (+) 1290 WP_000744767.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  NRG857_RS16670 (NRG857_16295) trkA 3453853..3455229 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  NRG857_RS16675 (NRG857_16300) mscL 3455359..3455772 (+) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40910.86 Da        Isoelectric Point: 6.0395

>NTDB_id=50029 NRG857_RS16650 WP_000228517.1 3449765..3450889(-) (dprA) [Escherichia coli O83:H1 str. NRG 857C]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=50029 NRG857_RS16650 WP_000228517.1 3449765..3450889(-) (dprA) [Escherichia coli O83:H1 str. NRG 857C]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment