Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   KIM14_RS25125 Genome accession   NZ_CP074895
Coordinates   4947619..4948743 (-) Length   374 a.a.
NCBI ID   WP_000228529.1    Uniprot ID   -
Organism   Escherichia coli strain 2009C_3689     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4942619..4953743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM14_RS25095 (KIM14_24795) yrdA 4944419..4944973 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  KIM14_RS25100 (KIM14_24800) yrdB 4944949..4945206 (-) 258 WP_001070563.1 DUF1488 domain-containing protein -
  KIM14_RS25105 (KIM14_24805) aroE 4945203..4946021 (-) 819 WP_000451211.1 shikimate dehydrogenase -
  KIM14_RS25110 (KIM14_24810) tsaC 4946026..4946598 (-) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  KIM14_RS25115 (KIM14_24815) yrdD 4946603..4947145 (-) 543 WP_001129719.1 type I DNA topoisomerase -
  KIM14_RS25120 (KIM14_24820) smg 4947174..4947647 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  KIM14_RS25125 (KIM14_24825) dprA 4947619..4948743 (-) 1125 WP_000228529.1 DNA-protecting protein DprA Machinery gene
  KIM14_RS25130 (KIM14_24830) def 4948873..4949382 (+) 510 WP_000114986.1 peptide deformylase -
  KIM14_RS25135 (KIM14_24835) fmt 4949397..4950344 (+) 948 WP_000004477.1 methionyl-tRNA formyltransferase -
  KIM14_RS25140 (KIM14_24840) rsmB 4950390..4951679 (+) 1290 WP_032345254.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  KIM14_RS25145 (KIM14_24845) trkA 4951701..4953077 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  KIM14_RS25150 (KIM14_24850) mscL 4953207..4953617 (+) 411 Protein_4916 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40926.86 Da        Isoelectric Point: 6.3122

>NTDB_id=494836 KIM14_RS25125 WP_000228529.1 4947619..4948743(-) (dprA) [Escherichia coli strain 2009C_3689]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=494836 KIM14_RS25125 WP_000228529.1 4947619..4948743(-) (dprA) [Escherichia coli strain 2009C_3689]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

44.865

98.93

0.444
  dprA Vibrio cholerae strain A1552

44.865

98.93

0.444
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Glaesserella parasuis strain SC1401

44.838

90.642

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393