Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   J0G24_RS04370 Genome accession   NZ_CP071259
Coordinates   889562..891055 (+) Length   497 a.a.
NCBI ID   WP_000249354.1    Uniprot ID   -
Organism   Escherichia coli strain MEI005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 884562..896055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G24_RS04350 (J0G24_04275) pilD 885210..886019 (+) 810 WP_001439205.1 prepilin peptidase PppA Machinery gene
  J0G24_RS04355 (J0G24_04280) gspS2 886085..886495 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  J0G24_RS04360 (J0G24_04285) gspC 886513..887472 (+) 960 WP_000135063.1 type II secretion system protein GspC -
  J0G24_RS04365 (J0G24_04290) gspD 887502..889562 (+) 2061 WP_000498824.1 type II secretion system secretin GspD -
  J0G24_RS04370 (J0G24_04295) pilB/pilB1 889562..891055 (+) 1494 WP_000249354.1 type II secretion system ATPase GspE Machinery gene
  J0G24_RS04375 (J0G24_04300) gspF 891055..892278 (+) 1224 WP_001173453.1 type II secretion system inner membrane protein GspF -
  J0G24_RS04380 (J0G24_04305) gspG 892295..892750 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  J0G24_RS04385 (J0G24_04310) gspH 892754..893317 (+) 564 WP_001115140.1 type II secretion system minor pseudopilin GspH -
  J0G24_RS04390 (J0G24_04315) gspI 893314..893685 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  J0G24_RS04395 (J0G24_04320) gspJ 893682..894281 (+) 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -
  J0G24_RS04400 (J0G24_04325) gspK 894284..895261 (+) 978 WP_000633238.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55190.33 Da        Isoelectric Point: 6.0390

>NTDB_id=476338 J0G24_RS04370 WP_000249354.1 889562..891055(+) (pilB/pilB1) [Escherichia coli strain MEI005]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEVRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=476338 J0G24_RS04370 WP_000249354.1 889562..891055(+) (pilB/pilB1) [Escherichia coli strain MEI005]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCGGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGGCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAGTCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

44.75

80.483

0.36