Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   JTC67_RS19305 Genome accession   NZ_CP070229
Coordinates   3812310..3813803 (+) Length   497 a.a.
NCBI ID   WP_000249338.1    Uniprot ID   -
Organism   Escherichia coli strain EC12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3807310..3818803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTC67_RS19285 (JTC67_19115) pilD 3807958..3808767 (+) 810 WP_001334345.1 prepilin peptidase PppA Machinery gene
  JTC67_RS19290 (JTC67_19120) gspS2 3808833..3809243 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  JTC67_RS19295 (JTC67_19125) gspC 3809261..3810220 (+) 960 WP_024242168.1 type II secretion system protein GspC -
  JTC67_RS19300 (JTC67_19130) gspD 3810250..3812310 (+) 2061 WP_000498844.1 type II secretion system secretin GspD -
  JTC67_RS19305 (JTC67_19135) pilB/pilB1 3812310..3813803 (+) 1494 WP_000249338.1 type II secretion system ATPase GspE Machinery gene
  JTC67_RS19310 (JTC67_19140) gspF 3813803..3815026 (+) 1224 WP_032344298.1 type II secretion system inner membrane protein GspF -
  JTC67_RS19315 (JTC67_19145) gspG 3815043..3815498 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  JTC67_RS19320 (JTC67_19150) gspH 3815502..3816065 (+) 564 WP_032344299.1 type II secretion system minor pseudopilin GspH -
  JTC67_RS19325 (JTC67_19155) gspI 3816062..3816433 (+) 372 WP_000820077.1 type II secretion system minor pseudopilin GspI -
  JTC67_RS19330 (JTC67_19160) gspJ 3816430..3817035 (+) 606 WP_021560036.1 type II secretion system minor pseudopilin GspJ -
  JTC67_RS19335 (JTC67_19165) gspK 3817032..3818009 (+) 978 WP_001357646.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55078.21 Da        Isoelectric Point: 5.7475

>NTDB_id=473670 JTC67_RS19305 WP_000249338.1 3812310..3813803(+) (pilB/pilB1) [Escherichia coli strain EC12]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCCQSAPA
TDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=473670 JTC67_RS19305 WP_000249338.1 3812310..3813803(+) (pilB/pilB1) [Escherichia coli strain EC12]
ATGGTACCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTACTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGTCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCCGCCTGGGGAG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTTTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCTGCCAGTCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

47.382

80.684

0.382
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362