Detailed information NTG0472651

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H5A40_RS20815
• Genome accession: NZ_CP059959
• Coordinates: 4624650..4625495 (-)
• Length: 281 a.a.
• NCBI ID: WP_040043677.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain IPO:4062 NAK:237
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4619650..4630495

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5A40_RS20790 (H5A40_20805)	-	4619852..4620622 (-)	771	WP_205591670.1	ABC transporter ATP-binding protein	-
H5A40_RS20795 (H5A40_20810)	-	4620624..4621661 (-)	1038	WP_205591671.1	FecCD family ABC transporter permease	-
H5A40_RS20800 (H5A40_20815)	-	4621658..4622722 (-)	1065	WP_205591672.1	ABC transporter substrate-binding protein	-
H5A40_RS20805 (H5A40_20820)	-	4623032..4624057 (+)	1026	WP_014916091.1	LLM class flavin-dependent oxidoreductase	-
H5A40_RS20810 (H5A40_20825)	ypdK	4624415..4624489 (+)	75	WP_099606229.1	membrane protein YpdK	-
H5A40_RS20815 (H5A40_20830)	pilD	4624650..4625495 (-)	846	WP_040043677.1	prepilin peptidase	Machinery gene
H5A40_RS20820 (H5A40_20835)	-	4625604..4626350 (-)	747	WP_205591673.1	type II secretion system protein N	-
H5A40_RS20825 (H5A40_20840)	gspM	4626340..4626834 (-)	495	WP_180785176.1	type II secretion system protein GspM	-
H5A40_RS20830 (H5A40_20845)	gspL	4626831..4628108 (-)	1278	WP_205591674.1	type II secretion system protein GspL	-
H5A40_RS20835 (H5A40_20850)	gspK	4628105..4629091 (-)	987	WP_014916095.1	type II secretion system minor pseudopilin GspK	-
H5A40_RS20840 (H5A40_20855)	gspJ	4629088..4629699 (-)	612	WP_014916096.1	type II secretion system minor pseudopilin GspJ	-
H5A40_RS20845 (H5A40_20860)	gspI	4629738..4630103 (-)	366	WP_039273738.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31439.39 Da
• Isoelectric Point: 5.7656

>NTDB_id=472651 H5A40_RS20815 WP_040043677.1 4624650..4625495(-) (pilD) [Pectobacterium brasiliense strain IPO:4062 NAK:237]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMVDSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=472651 H5A40_RS20815 WP_040043677.1 4624650..4625495(-) (pilD) [Pectobacterium brasiliense strain IPO:4062 NAK:237]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTTATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGATATCGAGCTTGAAACGG
GCATGGTTGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCGTGCCCGCACTGCCAGCAGGCTATT
GCGGTAAAAGATAACATCCCGCTATTCAGCTGGTTGTGGTTGCGCGGCCGCTCCCGCTGCTGTCATCAATCGGTGTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGTTACCCGGGATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATCAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAATGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGCAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTCGTGACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.185	96.441	0.484
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402