Detailed information NTG0472627

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H5A29_RS17250
• Genome accession: NZ_CP059958
• Coordinates: 3892789..3893634 (+)
• Length: 281 a.a.
• NCBI ID: WP_119871904.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain IPO:4065 NAK:223
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3887789..3898634

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5A29_RS17220 (H5A29_17195)	gspI	3888181..3888546 (+)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-
H5A29_RS17225 (H5A29_17200)	gspJ	3888546..3889196 (+)	651	WP_119871900.1	type II secretion system minor pseudopilin GspJ	-
H5A29_RS17230 (H5A29_17205)	gspK	3889193..3890179 (+)	987	WP_014916095.1	type II secretion system minor pseudopilin GspK	-
H5A29_RS17235 (H5A29_17210)	gspL	3890176..3891453 (+)	1278	WP_119871901.1	type II secretion system protein GspL	-
H5A29_RS17240 (H5A29_17215)	gspM	3891450..3891944 (+)	495	WP_119871902.1	type II secretion system protein GspM	-
H5A29_RS17245 (H5A29_17220)	-	3891934..3892680 (+)	747	WP_119871903.1	type II secretion system protein N	-
H5A29_RS17250 (H5A29_17225)	pilD	3892789..3893634 (+)	846	WP_119871904.1	prepilin peptidase	Machinery gene
H5A29_RS17255 (H5A29_17230)	ypdK	3893795..3893869 (-)	75	WP_099606229.1	membrane protein YpdK	-
H5A29_RS17260 (H5A29_17235)	-	3894227..3895252 (-)	1026	WP_014916091.1	LLM class flavin-dependent oxidoreductase	-
H5A29_RS17265 (H5A29_17240)	-	3895562..3896626 (+)	1065	WP_119871905.1	ABC transporter substrate-binding protein	-
H5A29_RS17270 (H5A29_17245)	-	3896623..3897660 (+)	1038	WP_119871906.1	FecCD family ABC transporter permease	-
H5A29_RS17275 (H5A29_17250)	-	3897662..3898432 (+)	771	WP_119871907.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31466.46 Da
• Isoelectric Point: 6.4557

>NTDB_id=472627 H5A29_RS17250 WP_119871904.1 3892789..3893634(+) (pilD) [Pectobacterium brasiliense strain IPO:4065 NAK:223]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSDPDKRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYVTGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=472627 H5A29_RS17250 WP_119871904.1 3892789..3893634(+) (pilD) [Pectobacterium brasiliense strain IPO:4065 NAK:223]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCATGGTGGTTTGGATCGCTGGGCGTGCTGGGATTGATTGTTGG
CAGTTTTTTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATGGCCGATTCCGATCCTGATAAGCGCTACAACCTGTGGTGGCCGCCTTCGTCGTGCCCACACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGTTGCGTGGCCGCTCCCGCTGCTGTCATCAATCAGTATCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTACCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAATGATGCCGTCGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGTAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTCGTGACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.185	96.441	0.484
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402