Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FY034_RS15030 Genome accession   NZ_CP058409
Coordinates   3238262..3239332 (+) Length   356 a.a.
NCBI ID   WP_265551961.1    Uniprot ID   -
Organism   Trichlorobacter lovleyi strain IAE     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3233262..3244332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FY034_RS15010 (FY034_15020) fmt 3233606..3234556 (+) 951 WP_265551953.1 methionyl-tRNA formyltransferase -
  FY034_RS15015 (FY034_15025) - 3234553..3235338 (+) 786 WP_265551954.1 DUF116 domain-containing protein -
  FY034_RS15020 (FY034_15030) - 3235451..3236452 (-) 1002 WP_265551957.1 hypothetical protein -
  FY034_RS15025 (FY034_15035) - 3236591..3238243 (+) 1653 WP_265551959.1 pilus assembly protein PilB -
  FY034_RS15030 (FY034_15040) pilT 3238262..3239332 (+) 1071 WP_265551961.1 type IV pilus twitching motility protein PilT Machinery gene
  FY034_RS15035 (FY034_15045) - 3239599..3241419 (-) 1821 WP_265551963.1 KUP/HAK/KT family potassium transporter -
  FY034_RS15040 (FY034_15050) - 3241500..3243320 (-) 1821 WP_265551965.1 KUP/HAK/KT family potassium transporter -
  FY034_RS15045 (FY034_15055) - 3243430..3244128 (-) 699 WP_265551967.1 response regulator -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39710.91 Da        Isoelectric Point: 6.9357

>NTDB_id=464140 FY034_RS15030 WP_265551961.1 3238262..3239332(+) (pilT) [Trichlorobacter lovleyi strain IAE]
MAKIDALFKMMKEQGASDLHLSSGNPPIFRQHGEMVRLQFKPLSHEELIPILYEILNDEQRAQFEATNDLDFAYAVPDLA
RFRGNIMMTHRGVAAVFRIIPAKILSADQLNLPEGVRRMTQFKKGLVLVTGPTGSGKSTTLAALIDLINATRKEHILTLE
DPLEFIHENKQSLLNQRQIGAHTQSFASALRAALREDPDIILVGEMRDLETIQLAMSAAETGHLVFGTLHTNTAAKTIDR
IIDVFPKESQDQVRTMLSESLKGVVCQQLLKTTDGHGRVAALEIMLGTPAIANLIREGKTFQIPSIIQTAKKDGMQLMDQ
HLLDLLKTRQINPEEAHRCAIDKKQFEQYLPQPTAT

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=464140 FY034_RS15030 WP_265551961.1 3238262..3239332(+) (pilT) [Trichlorobacter lovleyi strain IAE]
ATGGCCAAAATAGACGCACTCTTTAAAATGATGAAAGAGCAGGGGGCATCAGATCTGCACCTGTCATCCGGCAATCCGCC
CATCTTCCGCCAGCATGGCGAGATGGTCCGGCTGCAGTTCAAACCGCTCTCCCACGAAGAGTTGATCCCGATCCTGTATG
AGATCCTCAATGATGAGCAGCGGGCCCAGTTCGAGGCCACCAACGACCTTGACTTTGCCTACGCCGTGCCGGATCTGGCC
CGGTTCCGGGGCAACATCATGATGACCCACCGCGGCGTGGCAGCGGTCTTCAGGATCATCCCGGCCAAGATTCTCTCGGC
AGACCAGTTGAACCTGCCGGAGGGGGTGCGGCGGATGACCCAGTTCAAGAAAGGGCTGGTGCTGGTGACCGGCCCGACCG
GTTCAGGCAAATCCACCACCCTGGCAGCCCTGATCGATCTGATCAACGCCACCCGCAAGGAACATATCCTGACCCTGGAA
GACCCGCTGGAGTTCATCCATGAAAACAAACAGTCCCTGCTGAACCAGCGCCAGATCGGGGCACATACCCAGTCCTTTGC
CTCGGCCCTGCGGGCCGCCCTGCGGGAAGACCCGGACATCATCCTGGTGGGAGAGATGCGTGATCTGGAGACGATCCAGC
TGGCCATGAGCGCCGCCGAAACCGGTCACCTGGTCTTCGGCACCCTGCACACCAACACCGCGGCCAAGACGATTGACCGG
ATTATTGATGTCTTTCCCAAGGAATCCCAGGATCAGGTCCGCACCATGCTGTCGGAATCGCTCAAGGGGGTGGTCTGCCA
GCAACTGCTCAAGACCACTGACGGCCACGGGCGGGTGGCTGCGCTGGAGATCATGCTGGGGACCCCGGCCATTGCCAACC
TGATCCGCGAGGGCAAGACCTTCCAGATCCCCTCCATCATCCAGACCGCCAAGAAGGACGGCATGCAGCTGATGGACCAG
CACCTGCTGGACCTGCTCAAGACCAGGCAGATCAACCCGGAAGAGGCCCACCGCTGCGCCATTGACAAGAAACAGTTTGA
ACAGTACCTACCGCAACCGACTGCAACATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

58.61

92.978

0.545
  pilT Legionella pneumophila strain ERS1305867

58.61

92.978

0.545
  pilT Acinetobacter baylyi ADP1

56.268

96.348

0.542
  pilT Pseudomonas stutzeri DSM 10701

55.394

96.348

0.534
  pilT Acinetobacter baumannii D1279779

54.755

97.472

0.534
  pilT Acinetobacter baumannii strain A118

54.755

97.472

0.534
  pilT Acinetobacter nosocomialis M2

54.755

97.472

0.534
  pilT Pseudomonas aeruginosa PAK

55.786

94.663

0.528
  pilT Neisseria gonorrhoeae MS11

54.734

94.944

0.52
  pilT Neisseria meningitidis 8013

54.734

94.944

0.52
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.907

96.629

0.511
  pilT Vibrio cholerae strain A1552

52.907

96.629

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

96.91

0.489
  pilU Vibrio cholerae strain A1552

41.433

90.169

0.374