NTDB

Overview

Name	pilQ	Type	Machinery gene
Locus tag	I5P57_RS01665	Genome accession	NZ_CP066740
Coordinates	368449..369687 (+)	Length	412 a.a.
NCBI ID	WP_071595741.1	Uniprot ID	-
Organism	Escherichia coli strain R12
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 363449..374687

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5P57_RS01640 (I5P57_00326)	mrcA	363729..366281 (-)	2553	WP_001296475.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-
I5P57_RS01645 (I5P57_00327)	hofM	366401..367180 (+)	780	WP_001296474.1	DNA utilization protein HofM	-
I5P57_RS01650 (I5P57_00328)	hofN	367180..367719 (+)	540	WP_001069336.1	DNA utilization protein HofN	-
I5P57_RS01655 (I5P57_00329)	hofO	367703..368143 (+)	441	WP_001055743.1	DNA utilization protein HofO	-
I5P57_RS01660 (I5P57_00330)	hofP	368133..368537 (+)	405	WP_032286839.1	DNA utilization protein HofP	-
I5P57_RS01665 (I5P57_00331)	pilQ	368449..369687 (+)	1239	WP_071595741.1	DNA uptake porin HofQ	Machinery gene
I5P57_RS01670 (I5P57_00332)	aroK	370088..370609 (+)	522	WP_000818618.1	shikimate kinase AroK	-
I5P57_RS01675 (I5P57_00333)	aroB	370666..371754 (+)	1089	WP_000439850.1	3-dehydroquinate synthase	-
I5P57_RS01680 (I5P57_00334)	damX	371846..373132 (+)	1287	WP_000343172.1	cell division protein DamX	-
I5P57_RS01685 (I5P57_00335)	dam	373239..374075 (+)	837	WP_000742141.1	adenine-specific DNA-methyltransferase	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44795.29 Da Isoelectric Point: 5.9488

                                >NTDB_id=455780 I5P57_RS01665 WP_071595741.1 368449..369687(+) (pilQ) [Escherichia coli strain R12]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL

ITRQEGNILSVHSVAWQNDNIARQEAEQVRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=455780 I5P57_RS01665 WP_071595741.1 368449..369687(+) (pilQ) [Escherichia coli strain R12]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG

ATAACGCGGCAGGAAGGCAATATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA

GCAGGTGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG

GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGTTGGATATTATCGCCAGTCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTAGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilQ	Vibrio campbellii strain DS40M4	38.443	100	0.396
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
comE	Haemophilus influenzae 86-028NP	36.927	100	0.391
pilQ	Vibrio cholerae strain A1552	37.772	100	0.379
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.379
pilQ	Pseudomonas aeruginosa PAK	34.633	100	0.367
comE	Glaesserella parasuis strain SC1401	35.545	100	0.364

Detailed information NTG0455780