Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HUO05_RS11300 Genome accession   NZ_CP054700
Coordinates   2421002..2421595 (-) Length   197 a.a.
NCBI ID   WP_005381322.1    Uniprot ID   A0AAW9BKV9
Organism   Vibrio alginolyticus strain GS_MYPK1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2416002..2426595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUO05_RS11280 (HUO05_11280) aroB 2416888..2417988 (-) 1101 WP_149191989.1 3-dehydroquinate synthase -
  HUO05_RS11285 (HUO05_11285) aroK 2418020..2418538 (-) 519 WP_005381319.1 shikimate kinase AroK -
  HUO05_RS11290 (HUO05_11290) pilQ 2418726..2420471 (-) 1746 WP_165439071.1 type IV pilus secretin PilQ Machinery gene
  HUO05_RS11295 (HUO05_11295) pilP 2420497..2421012 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  HUO05_RS11300 (HUO05_11300) pilO 2421002..2421595 (-) 594 WP_005381322.1 type 4a pilus biogenesis protein PilO Machinery gene
  HUO05_RS11305 (HUO05_11305) pilN 2421588..2422163 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  HUO05_RS11310 (HUO05_11310) pilM 2422147..2423163 (-) 1017 WP_017633531.1 type IV pilus biogenesis protein PilM Machinery gene
  HUO05_RS11315 (HUO05_11315) - 2423308..2425869 (+) 2562 WP_005381325.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23015.53 Da        Isoelectric Point: 5.0885

>NTDB_id=454031 HUO05_RS11300 WP_005381322.1 2421002..2421595(-) (pilO) [Vibrio alginolyticus strain GS_MYPK1]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=454031 HUO05_RS11300 WP_005381322.1 2421002..2421595(-) (pilO) [Vibrio alginolyticus strain GS_MYPK1]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATTCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGTGCTAATACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.368

96.447

0.843
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685