Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   FOC87_RS03005 Genome accession   NZ_CP053981
Coordinates   545119..546468 (-) Length   449 a.a.
NCBI ID   WP_000225366.1    Uniprot ID   A0A158RMK4
Organism   Bacillus thuringiensis strain FDAARGOS_793     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540119..551468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOC87_RS02985 (FOC87_02985) secA 540441..542948 (-) 2508 WP_000579383.1 preprotein translocase subunit SecA -
  FOC87_RS02990 (FOC87_02990) raiA 543225..543767 (-) 543 WP_000671189.1 ribosome-associated translation inhibitor RaiA -
  FOC87_RS02995 (FOC87_02995) cspC 544091..544288 (-) 198 WP_001990088.1 cold shock protein CspC -
  FOC87_RS03000 (FOC87_03000) comFC 544415..545119 (-) 705 WP_003162242.1 comF operon protein ComFC -
  FOC87_RS03005 (FOC87_03005) comFA 545119..546468 (-) 1350 WP_000225366.1 ATP-dependent helicase ComFA Machinery gene
  FOC87_RS03010 (FOC87_03010) - 546596..547906 (-) 1311 WP_000734330.1 NlpC/P60 family protein -
  FOC87_RS03015 (FOC87_03015) - 548160..548474 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  FOC87_RS03020 (FOC87_03020) - 548648..549490 (-) 843 WP_000844766.1 DegV family protein -
  FOC87_RS03025 (FOC87_03025) - 549730..550365 (+) 636 WP_000926685.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51428.10 Da        Isoelectric Point: 9.6542

>NTDB_id=449647 FOC87_RS03005 WP_000225366.1 545119..546468(-) (comFA) [Bacillus thuringiensis strain FDAARGOS_793]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGTLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKEERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=449647 FOC87_RS03005 WP_000225366.1 545119..546468(-) (comFA) [Bacillus thuringiensis strain FDAARGOS_793]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTCATGAAAGAAAAGGAGAAAGAGAATTACATTCGTTACAGTGGAAAGGAACTTT
GTCTACTGGTCAGGAGTTGGCAGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCGGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGAAGAAAGAGTTTGTATTGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTCC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCGGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCATCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTGAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(312-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A158RMK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.914

93.096

0.483
  comFA Latilactobacillus sakei subsp. sakei 23K

40.5

89.087

0.361