Detailed information NTG0444642

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HNO52_RS19480
• Genome accession: NZ_CP053382
• Coordinates: 4186172..4187314 (-)
• Length: 380 a.a.
• NCBI ID: WP_197566821.1
• Uniprot ID: -
• Organism: Halomonas sp. MCCC 1A13316
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4181172..4192314

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HNO52_RS19470 (HNO52_19460)	tyrS	4183712..4184911 (-)	1200	WP_197566819.1	tyrosine--tRNA ligase	-
HNO52_RS19475 (HNO52_19465)	-	4185026..4186156 (+)	1131	WP_197566820.1	anhydro-N-acetylmuramic acid kinase	-
HNO52_RS19480 (HNO52_19470)	pilU	4186172..4187314 (-)	1143	WP_197566821.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HNO52_RS19485 (HNO52_19475)	pilT	4187345..4188379 (-)	1035	WP_197566822.1	type IV pilus twitching motility protein PilT	Machinery gene
HNO52_RS19490 (HNO52_19480)	-	4188458..4189168 (+)	711	WP_197569309.1	YggS family pyridoxal phosphate-dependent enzyme	-
HNO52_RS19495 (HNO52_19485)	proC	4189228..4190052 (+)	825	WP_197566823.1	pyrroline-5-carboxylate reductase	-
HNO52_RS19500 (HNO52_19490)	-	4190112..4190702 (+)	591	WP_197566824.1	YggT family protein	-
HNO52_RS19505 (HNO52_19495)	-	4190754..4191911 (+)	1158	WP_197569310.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42663.75 Da
• Isoelectric Point: 6.0978

>NTDB_id=444642 HNO52_RS19480 WP_197566821.1 4186172..4187314(-) (pilU) [Halomonas sp. MCCC 1A13316]
MKAQEWLEQLLDIMAQQEASDLLISVQAPPTIKTAGKLTALGDQRLSVEQVRELVNTAVPEGLRERFLQEREANFALSLS
GRGRFRVSAFQQRNQWAMVIRRIAYEIPHLEELSLPAQLGELANMKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHKKAIINQREVGIDTESFEVALKNTLRQAPDVVLIGEVRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNLHAIVAQQLLPTRDGGRTPAIEILLRTPLIADLIRKGDVGEIKNVMARSRDQGMQTF
DQALFELYKAGKISEQVAMVHADSANELRMMIKYGDEKSDVLAKAQDSAQRFSLREDDDF

Nucleotide

• Length: 1143 bp

>NTDB_id=444642 HNO52_RS19480 WP_197566821.1 4186172..4187314(-) (pilU) [Halomonas sp. MCCC 1A13316]
ATGAAAGCACAGGAATGGTTGGAGCAGTTGCTCGACATCATGGCCCAGCAGGAGGCCTCGGATCTGCTGATCTCGGTGCA
AGCACCGCCGACGATCAAGACCGCAGGAAAGCTCACGGCACTAGGCGACCAGCGGCTTTCGGTGGAGCAGGTGCGCGAGC
TGGTCAATACAGCGGTGCCCGAGGGGCTTCGTGAGCGTTTCCTGCAGGAGCGTGAGGCCAACTTCGCGCTGAGCCTCAGC
GGCCGGGGACGTTTCCGGGTCAGCGCCTTTCAGCAGCGCAACCAGTGGGCCATGGTCATCCGGCGCATTGCTTACGAGAT
CCCTCATCTCGAGGAGCTCTCGCTGCCGGCGCAGTTGGGGGAGCTGGCCAATATGAAGCGCGGGCTGGTGTTCGTGGTGG
GCGGTACCGGCACCGGCAAGTCGACTACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACCCTGGGCGGACATATCATC
AGCGTCGAGGACCCCATCGAGTACGTGCACCCGCACAAGAAGGCGATTATCAACCAGCGCGAAGTGGGCATCGATACCGA
ATCCTTCGAGGTGGCGCTGAAGAATACTCTGCGCCAGGCGCCGGACGTCGTCCTGATCGGCGAGGTGCGAACCCGCGAGA
CCATGGAGCATGCGCTGACCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCATGCCAACAACGCCAACCAGGCG
CTGGATCGCATCATCCACTTCTTCCCGCATGAGCGCCACGAGCAGATCTGGCTCGACCTGTCGCTCAACCTGCACGCCAT
CGTCGCCCAGCAGCTCCTGCCGACTCGCGATGGGGGACGCACGCCGGCCATCGAGATCCTGCTGCGCACGCCGCTGATCG
CCGACCTGATTCGCAAGGGCGACGTGGGCGAGATCAAGAACGTCATGGCTCGCTCGCGCGACCAGGGCATGCAGACCTTC
GATCAAGCGCTGTTCGAACTCTACAAGGCCGGCAAGATCAGCGAGCAGGTAGCCATGGTGCACGCCGACTCGGCCAACGA
GCTGCGCATGATGATCAAGTACGGTGACGAGAAGAGCGACGTGCTGGCCAAGGCGCAGGACAGCGCGCAGCGCTTCTCCC
TGCGCGAGGATGATGATTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (16-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.932	92.632	0.574
pilU	Vibrio cholerae strain A1552	58.908	91.579	0.539
pilU	Acinetobacter baylyi ADP1	54.645	96.316	0.526
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.679	85.263	0.389
pilT	Neisseria meningitidis 8013	41.742	87.632	0.366
pilT	Neisseria gonorrhoeae MS11	41.441	87.632	0.363
pilT	Legionella pneumophila strain ERS1305867	41.265	87.368	0.361
pilT	Legionella pneumophila strain Lp02	41.265	87.368	0.361