Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   IUJ43_RS21280 Genome accession   NZ_CP064391
Coordinates   4639278..4639874 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4634278..4644874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ43_RS21260 (IUJ43_21205) aroK 4635409..4635927 (-) 519 WP_003095833.1 shikimate kinase AroK -
  IUJ43_RS21265 (IUJ43_21210) pilQ 4635939..4638083 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  IUJ43_RS21270 (IUJ43_21215) comL/pilP 4638137..4638661 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  IUJ43_RS21275 (IUJ43_21220) pilO 4638658..4639281 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  IUJ43_RS21280 (IUJ43_21225) pilN 4639278..4639874 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  IUJ43_RS21285 (IUJ43_21230) pilM 4639874..4640938 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  IUJ43_RS21290 (IUJ43_21235) - 4641123..4643591 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=443684 IUJ43_RS21280 WP_003095839.1 4639278..4639874(-) (pilN) [Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=443684 IUJ43_RS21280 WP_003095839.1 4639278..4639874(-) (pilN) [Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369