Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   HKM25_RS09840 Genome accession   NZ_CP053210
Coordinates   1929360..1930184 (-) Length   274 a.a.
NCBI ID   WP_001036789.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 6A-10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1924360..1935184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKM25_RS09835 (HKM25_2016) - 1927738..1929069 (-) 1332 WP_000390043.1 hemolysin family protein -
  HKM25_RS09840 (HKM25_2017) endA 1929360..1930184 (-) 825 WP_001036789.1 DNA-entry nuclease EndA Machinery gene
  HKM25_RS09845 (HKM25_2018) - 1930223..1930411 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  HKM25_RS09850 (HKM25_2019) murA 1930404..1931687 (-) 1284 WP_000358024.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  HKM25_RS09855 (HKM25_2020) - 1931711..1931941 (-) 231 WP_000250394.1 DUF1146 family protein -
  HKM25_RS09860 (HKM25_2021) sepM 1931999..1933036 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  HKM25_RS09865 (HKM25_2022) coaD 1933020..1933508 (-) 489 WP_001280745.1 pantetheine-phosphate adenylyltransferase -
  HKM25_RS09870 (HKM25_2023) rsmD 1933498..1934037 (-) 540 WP_000706963.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  HKM25_RS09875 (HKM25_2024) asnA 1934102..1935094 (-) 993 WP_000747990.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29966.67 Da        Isoelectric Point: 10.1700

>NTDB_id=443467 HKM25_RS09840 WP_001036789.1 1929360..1930184(-) (endA) [Streptococcus pneumoniae strain 6A-10]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLNQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=443467 HKM25_RS09840 WP_001036789.1 1929360..1930184(-) (endA) [Streptococcus pneumoniae strain 6A-10]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAATCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.175

100

0.982
  endA Streptococcus pneumoniae D39

98.175

100

0.982
  endA Streptococcus pneumoniae R6

98.175

100

0.982
  endA Streptococcus pneumoniae TIGR4

98.175

100

0.982