Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   HLB22_RS07720 Genome accession   NZ_CP053101
Coordinates   1616862..1617359 (-) Length   165 a.a.
NCBI ID   WP_029697681.1    Uniprot ID   A0AAE2ZZ83
Organism   Staphylococcus aureus strain EDCC5398     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1611862..1622359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLB22_RS07695 (HLB22_07695) gcvPB 1612024..1613496 (-) 1473 WP_000202191.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  HLB22_RS07700 (HLB22_07700) gcvPA 1613489..1614835 (-) 1347 WP_000019688.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  HLB22_RS07705 (HLB22_07705) gcvT 1614855..1615946 (-) 1092 WP_000093355.1 glycine cleavage system aminomethyltransferase GcvT -
  HLB22_RS07710 (HLB22_07710) - 1616105..1616629 (-) 525 WP_001015122.1 shikimate kinase -
  HLB22_RS07715 (HLB22_07715) - 1616619..1616765 (-) 147 WP_001789879.1 hypothetical protein -
  HLB22_RS07720 (HLB22_07720) comGF 1616862..1617359 (-) 498 WP_029697681.1 competence type IV pilus minor pilin ComGF Machinery gene
  HLB22_RS07725 (HLB22_07725) comGE 1617277..1617576 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  HLB22_RS07730 (HLB22_07730) comGD 1617563..1618009 (-) 447 WP_075583651.1 competence type IV pilus minor pilin ComGD Machinery gene
  HLB22_RS07735 (HLB22_07735) comGC 1617987..1618298 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  HLB22_RS07740 (HLB22_07740) comGB 1618312..1619382 (-) 1071 WP_000776403.1 competence type IV pilus assembly protein ComGB Machinery gene
  HLB22_RS07745 (HLB22_07745) comGA 1619354..1620328 (-) 975 WP_000697215.1 competence type IV pilus ATPase ComGA Machinery gene
  HLB22_RS07750 (HLB22_07750) - 1620380..1621003 (-) 624 WP_001223006.1 MBL fold metallo-hydrolase -
  HLB22_RS07755 (HLB22_07755) - 1621000..1621329 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  HLB22_RS07760 (HLB22_07760) - 1621329..1622315 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19211.94 Da        Isoelectric Point: 10.2436

>NTDB_id=443032 HLB22_RS07720 WP_029697681.1 1616862..1617359(-) (comGF) [Staphylococcus aureus strain EDCC5398]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFRGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=443032 HLB22_RS07720 WP_029697681.1 1616862..1617359(-) (comGF) [Staphylococcus aureus strain EDCC5398]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAGAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(69-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

97.576

100

0.976