Detailed information NTG0442037

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: IRJ27_RS07075
• Genome accession: NZ_CP064095
• Coordinates: 1410901..1412076 (-)
• Length: 391 a.a.
• NCBI ID: WP_009966637.1
• Uniprot ID: P96649
• Organism: Bacillus subtilis strain N1303-2Ay
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1405901..1417076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IRJ27_RS07045	yddQ	1406224..1406766 (-)	543	WP_003234256.1	cysteine hydrolase family protein	-
IRJ27_RS07050	lrpB	1406881..1407330 (+)	450	WP_003246612.1	transcriptional regulator LrpB	-
IRJ27_RS07055	lrpA	1407453..1407863 (-)	411	WP_003246601.1	transcriptional regulator LrpA	-
IRJ27_RS07060	yddN	1408123..1409142 (+)	1020	WP_003234259.1	LLM class flavin-dependent oxidoreductase	-
IRJ27_RS07065	yddM	1409731..1410672 (-)	942	WP_010886416.1	helicase	-
IRJ27_RS07070	phrI	1410825..1410944 (-)	120	WP_010886415.1	phosphatase RapI inhibitor PhrI	-
IRJ27_RS07075	rapF	1410901..1412076 (-)	1176	WP_009966637.1	response regulator aspartate phosphatase RapI	Regulator
IRJ27_RS07080	yddK	1412416..1413216 (+)	801	WP_009966636.1	toll/interleukin-1 receptor domain-containing protein	-
IRJ27_RS07085	yddJ	1413407..1413787 (-)	381	WP_009969670.1	DUF4467 domain-containing protein	-
IRJ27_RS07090	-	1413850..1414356 (-)	507	WP_009966634.1	hypothetical protein	-
IRJ27_RS07095	cwlT	1414371..1415360 (-)	990	WP_009966633.1	bifunctional lytic transglycosylase/C40 family peptidase	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 46566.15 Da
• Isoelectric Point: 4.9688

>NTDB_id=442037 IRJ27_RS07075 WP_009966637.1 1410901..1412076(-) (rapF) [Bacillus subtilis strain N1303-2Ay]
MRGVFLDKDKIPYDLVTKKLNEWYTSIKNDQVEQAEIIKTEVEKELLNMEENQDALLYYQLLEFRHEIMLSYMKSKEIED
LNNAYETIKEIEKQGQLTGMLEYYFYFFKGMYEFRRKELISAISAYRIAESKLSEVEDEIEKAEFFFKVSYVYYYMKQTY
FSMNYANRALKIFREYEEYAVQTVRCQFIVAGNLIDSLEYERALEQFLKSLEISKESNIEHLIAMSHMNIGICYDELKEY
KKASQHLILALEIFEKSKHSFLTKTLFTLTYVEAKQQNYNVALIYFRKGRFIADKSDDKEYSAKFKILEGLFFSDGETQL
IKNAFSYLASRKMFADVENFSIEVADYFHEQGNLMLSNEYYRMSIEARRKIKKGEIIDENQPDSIGSSDFK

Nucleotide

• Length: 1176 bp

>NTDB_id=442037 IRJ27_RS07075 WP_009966637.1 1410901..1412076(-) (rapF) [Bacillus subtilis strain N1303-2Ay]
TTGCGGGGTGTTTTCTTAGATAAAGATAAAATTCCGTACGACTTAGTCACGAAAAAGTTAAATGAATGGTATACATCAAT
AAAAAATGATCAAGTTGAGCAAGCCGAGATTATAAAAACAGAAGTAGAGAAAGAATTGTTAAACATGGAAGAAAATCAAG
ATGCCCTGTTATATTATCAACTATTAGAATTTAGACATGAGATAATGCTGAGTTATATGAAATCTAAGGAAATAGAAGAT
CTCAATAATGCTTATGAGACTATAAAAGAAATTGAGAAGCAAGGGCAATTAACTGGCATGTTGGAATACTATTTTTACTT
TTTTAAGGGTATGTACGAGTTTAGGCGTAAAGAATTAATTTCAGCGATAAGTGCTTATCGAATAGCTGAATCAAAGTTGT
CAGAAGTTGAGGATGAAATAGAGAAAGCAGAGTTTTTTTTCAAAGTGTCCTATGTATATTATTATATGAAACAAACATAC
TTCTCCATGAATTATGCAAATCGTGCACTCAAAATATTTAGAGAGTATGAAGAATATGCTGTCCAGACTGTGCGTTGTCA
ATTTATTGTAGCAGGAAACTTGATCGATTCATTGGAATATGAAAGAGCCTTGGAACAATTTTTGAAGTCTTTGGAAATTT
CCAAGGAAAGTAACATAGAGCATTTAATTGCAATGTCACATATGAATATTGGGATTTGTTATGATGAATTGAAAGAATAT
AAGAAGGCTTCACAACATTTAATTTTAGCGTTAGAAATTTTTGAAAAATCAAAACATAGTTTCTTAACAAAGACTTTATT
CACTCTAACCTATGTAGAAGCAAAACAACAAAATTATAATGTTGCTTTGATATACTTTAGGAAAGGGCGATTTATTGCCG
ATAAAAGTGATGATAAGGAATACTCAGCGAAATTCAAAATATTAGAGGGATTATTTTTTTCTGATGGTGAGACTCAATTA
ATAAAGAATGCATTTTCATATCTGGCTTCGAGAAAAATGTTTGCTGATGTTGAAAATTTTTCGATTGAAGTCGCTGATTA
TTTTCATGAACAAGGAAATTTAATGCTCTCTAATGAATATTATCGTATGAGTATTGAAGCAAGACGAAAAATTAAAAAAG
GGGAGATTATTGATGAAAATCAGCCGGATTCTATTGGCAGCAGTGATTTTAAGTAG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (10-70)

• Tetratricopeptide repeat (TPR_12) (199-256)

3D structure

• PDB: 4I1A

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	38.525	93.606	0.361
rapC	Bacillus subtilis subsp. subtilis str. 168	38.108	94.629	0.361