Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FTU_RS00455 Genome accession   NC_016933
Coordinates   91696..92724 (+) Length   342 a.a.
NCBI ID   WP_003019818.1    Uniprot ID   A0AAD3AVU4
Organism   Francisella tularensis subsp. tularensis TIGB03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 92731..135064 91696..92724 flank 7


Gene organization within MGE regions


Location: 91696..135064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FTU_RS00455 (FTU_0084) pilT 91696..92724 (+) 1029 WP_003019818.1 type IV pilus twitching motility protein PilT Machinery gene
  FTU_RS10290 - 92788..93243 (-) 456 WP_003019819.1 hypothetical protein -
  FTU_RS10295 - 93305..93463 (-) 159 WP_158087690.1 hypothetical protein -
  FTU_RS10300 - 93517..93789 (-) 273 WP_003023016.1 NAD(P)-binding protein -
  FTU_RS00465 (FTU_0086) - 93786..94232 (-) 447 WP_003019821.1 DUF3429 domain-containing protein -
  FTU_RS00470 (FTU_0087) - 94243..95205 (-) 963 WP_003019823.1 cytochrome d ubiquinol oxidase subunit II -
  FTU_RS00475 (FTU_0088) - 95209..96585 (-) 1377 Protein_94 cytochrome ubiquinol oxidase subunit I -
  FTU_RS00480 (FTU_0089) - 96821..97702 (+) 882 WP_003019824.1 TIGR01777 family oxidoreductase -
  FTU_RS00485 (FTU_0090) - 97699..99126 (-) 1428 WP_003019826.1 HAMP domain-containing sensor histidine kinase -
  FTU_RS00490 (FTU_0091) - 99242..100198 (+) 957 WP_003019829.1 hypothetical protein -
  FTU_RS00500 (FTU_0092) - 100440..101480 (+) 1041 WP_003023028.1 ATP-grasp domain-containing protein -
  FTU_RS00505 (FTU_0093) - 101638..102159 (+) 522 WP_003019831.1 hypothetical protein -
  FTU_RS00515 - 103119..103862 (+) 744 WP_003019836.1 IS5-like element ISFtu2 family transposase -
  FTU_RS00520 - 103890..104303 (+) 414 Protein_102 IS1595-like element ISFtu3 family transposase -
  FTU_RS00525 (FTU_0094) ldcA 104532..105545 (+) 1014 WP_003019840.1 LD-carboxypeptidase LdcA -
  FTU_RS00530 - 106196..107052 (+) 857 Protein_104 IS982-like element ISFtu4 family transposase -
  FTU_RS00535 (FTU_0095) - 106990..107571 (-) 582 WP_003019536.1 transposase -
  FTU_RS00540 (FTU_0096) - 107816..109093 (-) 1278 WP_003019842.1 MFS transporter -
  FTU_RS00545 (FTU_0097) - 109225..112338 (-) 3114 WP_003023045.1 efflux RND transporter permease subunit -
  FTU_RS00550 (FTU_0098) - 112338..113711 (-) 1374 WP_014367898.1 efflux RND transporter periplasmic adaptor subunit -
  FTU_RS00555 (FTU_0099) - 113724..114215 (-) 492 WP_003017112.1 disulfide bond formation protein B -
  FTU_RS00560 (FTU_0100) - 114255..115337 (-) 1083 WP_003019848.1 CCA tRNA nucleotidyltransferase -
  FTU_RS00565 (FTU_0101) msbA 115442..117271 (+) 1830 WP_014367899.1 lipid A export permease/ATP-binding protein MsbA -
  FTU_RS00570 (FTU_0102) lpxK 117277..118245 (+) 969 WP_003019528.1 tetraacyldisaccharide 4'-kinase -
  FTU_RS00575 (FTU_0103) polA 118341..121034 (+) 2694 WP_003019852.1 DNA polymerase I -
  FTU_RS00580 (FTU_0104) - 121054..121830 (+) 777 WP_003019854.1 type III pantothenate kinase -
  FTU_RS00585 (FTU_0105) - 121835..123079 (+) 1245 WP_003019856.1 phosphopentomutase -
  FTU_RS00590 (FTU_0106) deoC 123042..123803 (+) 762 WP_003019858.1 deoxyribose-phosphate aldolase -
  FTU_RS00595 (FTU_0107) - 123793..124992 (+) 1200 WP_003019860.1 nucleoside transporter C-terminal domain-containing protein -
  FTU_RS00600 (FTU_0108) - 125011..126213 (+) 1203 WP_003019862.1 nucleoside transporter C-terminal domain-containing protein -
  FTU_RS00605 (FTU_0109) tmk 126226..126855 (+) 630 WP_003019864.1 dTMP kinase -
  FTU_RS00610 (FTU_0110) - 126917..128494 (+) 1578 WP_003019866.1 peptide chain release factor 3 -
  FTU_RS00615 (FTU_0111) - 128569..129885 (+) 1317 WP_003019870.1 LysM peptidoglycan-binding domain-containing protein -
  FTU_RS00620 (FTU_0112) ftsY 129918..130913 (+) 996 WP_003024750.1 signal recognition particle-docking protein FtsY -
  FTU_RS00625 (FTU_0113) - 131004..133226 (+) 2223 WP_003019873.1 UvrD-helicase domain-containing protein -
  FTU_RS00630 (FTU_0114) - 133367..134824 (+) 1458 WP_003019875.1 peptide ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 38031.76 Da        Isoelectric Point: 6.8391

>NTDB_id=44080 FTU_RS00455 WP_003019818.1 91696..92724(+) (pilT) [Francisella tularensis subsp. tularensis TIGB03]
MIRKLLTLCVQKKASDLHLSSGCKAKYRIDGDLIDIESSPVLNDKMISQMLLEIMTDDQKDELIETYECDFSIDDRDNDA
RFRVNAFFHNRGYGAVFRRLENTIPTLDQFGAPRILKEVQAKKGGLILVTGPTGSGKSSTLAALVNEINHKEDSHILTIE
DPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDCILVGEMRDLETIRLALEAAETGHLVLGTLHTISAIKTVDR
VISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLSQIYTALQTGTAKGMATIEQS
IENLLKAGTITPEEAAKYIVVR

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=44080 FTU_RS00455 WP_003019818.1 91696..92724(+) (pilT) [Francisella tularensis subsp. tularensis TIGB03]
ATGATAAGAAAATTACTAACTCTTTGTGTGCAAAAAAAAGCCTCTGATCTACATTTATCCTCTGGTTGTAAGGCTAAATA
TAGAATAGATGGAGATTTGATTGATATTGAATCTTCACCTGTATTAAATGATAAGATGATTTCTCAGATGCTTTTAGAAA
TCATGACAGATGATCAAAAAGATGAGCTGATAGAAACCTACGAATGTGATTTTTCTATAGATGATAGAGATAATGATGCG
AGATTTAGGGTAAATGCTTTTTTCCATAACCGTGGTTATGGCGCGGTATTTCGTCGTTTAGAGAATACTATTCCAACGTT
AGATCAATTTGGAGCACCAAGAATTCTTAAAGAAGTTCAAGCTAAAAAAGGTGGCTTAATTCTAGTAACAGGACCCACAG
GTTCAGGTAAGAGTAGTACATTGGCAGCATTAGTCAATGAAATAAATCATAAGGAAGATTCTCATATTCTAACTATTGAG
GATCCCGTAGAATTTGTTCATACAAGTCAAAGATCACTAGTAAACCAACGTGAAGTTAAAAGAGATACAAAAAGCTTTAA
TGCGGCTCTTAAATCAGCACTAAGGGAGGATCCTGATTGTATTCTAGTTGGAGAGATGCGTGATTTAGAAACTATTAGAT
TAGCACTAGAGGCAGCTGAAACTGGGCATTTGGTTTTAGGGACATTACATACAATATCAGCTATAAAAACTGTTGATAGA
GTAATATCTGTATTTCCACCTGCTGAGCAGGAGCTTGTACGCAACATGCTTGCGGAATCATTACAAATCGTTATATCTCA
ACGTTTACTTAAACGTAAGGGCGGCGGGCGTGTCGCAGCATATGAAGTTCTTATTTCAAATACTGGTATTAGAAATATGA
TTAAAGAAAATAAACTTTCGCAAATTTACACCGCACTACAAACTGGAACAGCTAAGGGAATGGCGACAATCGAACAGAGC
ATTGAGAATCTTCTTAAGGCTGGAACAATTACACCAGAAGAAGCCGCTAAATACATTGTAGTAAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

56.287

97.661

0.55
  pilT Acinetobacter baumannii strain A118

56.287

97.661

0.55
  pilT Acinetobacter nosocomialis M2

56.287

97.661

0.55
  pilT Legionella pneumophila strain Lp02

54.734

98.83

0.541
  pilT Legionella pneumophila strain ERS1305867

54.734

98.83

0.541
  pilT Pseudomonas aeruginosa PAK

54.79

97.661

0.535
  pilT Neisseria gonorrhoeae MS11

54.006

98.538

0.532
  pilT Neisseria meningitidis 8013

54.006

98.538

0.532
  pilT Acinetobacter baylyi ADP1

55.319

96.199

0.532
  pilT Pseudomonas stutzeri DSM 10701

54.192

97.661

0.529
  pilT Vibrio cholerae strain A1552

53.55

98.83

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.55

98.83

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.015

96.784

0.436
  pilU Acinetobacter baylyi ADP1

39.403

97.953

0.386
  pilU Pseudomonas stutzeri DSM 10701

39.039

97.368

0.38
  pilU Vibrio cholerae strain A1552

39.198

94.737

0.371

Multiple sequence alignment