Detailed information NTG0043743

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: B657_RS01705
• Genome accession: NC_018520
• Coordinates: 304442..305563 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis QB928
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 299442..310563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B657_RS01685 (B657_02780)	ycdA	299450..300514 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
B657_RS22335	-	300537..300668 (-)	132	WP_003246334.1	hypothetical protein	-
B657_RS01690 (B657_02790)	ycdB	300842..302260 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
B657_RS01695 (B657_02800)	ycdC	302447..303808 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
B657_RS01700 (B657_02810)	cwlK	303816..304319 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
B657_RS01705 (B657_02820)	rapF	304442..305563 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
B657_RS01710 (B657_02830)	ycdF	305670..306446 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
B657_RS01715 (B657_02840)	ycdG	306471..308156 (+)	1686	WP_003234736.1	alpha-glucosidase	-
B657_RS01720 (B657_02850)	znuA	308344..309303 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
B657_RS01725 (B657_02860)	znuC	309359..310054 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=43743 B657_RS01705 WP_003246478.1 304442..305563(+) (rapF) [Bacillus subtilis QB928]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=43743 B657_RS01705 WP_003246478.1 304442..305563(+) (rapF) [Bacillus subtilis QB928]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405