Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IMZ18_RS05225 Genome accession   NZ_CP063151
Coordinates   985032..985442 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain CMIN-4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 980032..990442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMZ18_RS05200 (IMZ18_05200) - 981137..982027 (-) 891 WP_014480326.1 LysR family transcriptional regulator -
  IMZ18_RS05205 (IMZ18_05205) fumC 982194..983582 (+) 1389 WP_014480325.1 class II fumarate hydratase -
  IMZ18_RS22445 - 983801..984013 (+) 213 Protein_1020 recombinase family protein -
  IMZ18_RS05215 (IMZ18_05215) - 984010..984108 (-) 99 WP_031600702.1 hypothetical protein -
  IMZ18_RS05220 (IMZ18_05220) sigK 984108..984836 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  IMZ18_RS05225 (IMZ18_05225) nucA/comI 985032..985442 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  IMZ18_RS05230 (IMZ18_05230) yqeB 985475..986197 (-) 723 WP_014480321.1 hypothetical protein -
  IMZ18_RS05235 (IMZ18_05235) gnd 986448..987341 (+) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  IMZ18_RS05240 (IMZ18_05240) yqeD 987360..987986 (-) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  IMZ18_RS05245 (IMZ18_05245) cwlH 988173..988925 (+) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  IMZ18_RS05250 (IMZ18_05250) yqeF 989177..989908 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  IMZ18_RS05255 (IMZ18_05255) - 990214..990354 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=437206 IMZ18_RS05225 WP_009967785.1 985032..985442(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain CMIN-4]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=437206 IMZ18_RS05225 WP_009967785.1 985032..985442(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain CMIN-4]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529