Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   G8B37_RS00680 Genome accession   NZ_CP049695
Coordinates   106575..107609 (+) Length   344 a.a.
NCBI ID   WP_257577523.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain ABC76     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 101575..112609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8B37_RS00670 (G8B37_00670) - 105243..105608 (+) 366 WP_002986560.1 DUF1033 family protein -
  G8B37_RS00675 (G8B37_00675) comYA 105701..106639 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  G8B37_RS00680 (G8B37_00680) comYB 106575..107609 (+) 1035 WP_257577523.1 competence type IV pilus assembly protein ComGB Machinery gene
  G8B37_RS00685 (G8B37_00685) comYC 107611..107937 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  G8B37_RS00690 (G8B37_00690) comGD 107912..108340 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  G8B37_RS00695 (G8B37_00695) comGE 108297..108581 (+) 285 WP_011528162.1 competence type IV pilus minor pilin ComGE -
  G8B37_RS00700 (G8B37_00700) comYF 108574..109008 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  G8B37_RS00705 (G8B37_00705) comGG 108992..109318 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  G8B37_RS00710 (G8B37_00710) comYH 109416..110369 (+) 954 WP_021299429.1 class I SAM-dependent methyltransferase Machinery gene
  G8B37_RS00715 (G8B37_00715) - 110428..111624 (+) 1197 WP_011528164.1 acetate kinase -
  G8B37_RS00720 (G8B37_00720) - 111811..112119 (+) 309 WP_011528165.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39662.12 Da        Isoelectric Point: 9.7085

>NTDB_id=427121 G8B37_RS00680 WP_257577523.1 106575..107609(+) (comYB) [Streptococcus pyogenes strain ABC76]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLVGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=427121 G8B37_RS00680 WP_257577523.1 106575..107609(+) (comYB) [Streptococcus pyogenes strain ABC76]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGTTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAGTAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.462

94.477

0.401