Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NH8B_RS17080 Genome accession   NC_016002
Coordinates   3684175..3685884 (-) Length   569 a.a.
NCBI ID   WP_014088351.1    Uniprot ID   G2IZF6
Organism   Pseudogulbenkiania sp. NH8B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3679175..3690884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH8B_RS20570 (NH8B_3508) yacG 3680404..3680598 (-) 195 WP_014088347.1 DNA gyrase inhibitor YacG -
  NH8B_RS17060 (NH8B_3509) zapD 3680607..3681365 (-) 759 WP_040652180.1 cell division protein ZapD -
  NH8B_RS17065 (NH8B_3510) coaE 3681452..3682072 (-) 621 WP_014088348.1 dephospho-CoA kinase -
  NH8B_RS17070 (NH8B_3511) pilD 3682059..3682925 (-) 867 WP_014088349.1 A24 family peptidase Machinery gene
  NH8B_RS17075 (NH8B_3512) pilC 3682925..3684151 (-) 1227 WP_014088350.1 type II secretion system F family protein Machinery gene
  NH8B_RS17080 (NH8B_3513) pilB 3684175..3685884 (-) 1710 WP_014088351.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NH8B_RS17085 (NH8B_3514) - 3686134..3687204 (-) 1071 WP_014088352.1 porin -
  NH8B_RS17090 (NH8B_3515) - 3687582..3688991 (+) 1410 WP_014088353.1 deoxyribodipyrimidine photo-lyase -
  NH8B_RS17095 (NH8B_3516) - 3689033..3689404 (-) 372 WP_014088354.1 ComEA family DNA-binding protein -
  NH8B_RS17100 (NH8B_3517) - 3689463..3690284 (-) 822 WP_014088355.1 inner membrane protein YpjD -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62311.78 Da        Isoelectric Point: 5.3341

>NTDB_id=42375 NH8B_RS17080 WP_014088351.1 3684175..3685884(-) (pilB) [Pseudogulbenkiania sp. NH8B]
MEVSAGVSGLGRALVQHNSLLLQDAEAIQRQAQSSSISFVEQLILSKKMSSLEVATFASQVFGFPLLDLAAIEPEQLPQG
LVDEDLISSRRLLPLYKRGSRLFIGTSDPTQTSAFHAITFKTSLSVEVVVVEDDKLGKLIGSYQAGGAEAIKALVDEDFG
DLEFAEPDAEPEKTVAQTEVDDAPVVKFIHKVLLDAINGGASDVHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASR
IKVISRLDISEKRVPQDGRLKLVLSKTRAIDFRVSTLPTLFGEKIVMRILDQSAATLNIEQLGFEPEQKAMLLEAISRPY
GMVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNERAGLTFSSALKAFLRQDPDVIMVGEIRD
FETADIAIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNVASSVLLIMAQRLARRLCNNCKKPMDIPKEALLRAGYR
EVELDGSWQPYGPVGCRECRDSGYRGRVGIYEVMPITDAMTRLIMKNGTAVDIADLARQEGMVDLRRAGLLKVKQGITSL
AEIEAATNE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=42375 NH8B_RS17080 WP_014088351.1 3684175..3685884(-) (pilB) [Pseudogulbenkiania sp. NH8B]
ATGGAAGTTTCTGCCGGCGTTTCCGGCCTCGGCCGAGCCTTGGTGCAACATAACAGCCTGCTGCTGCAGGATGCCGAGGC
TATTCAGCGCCAGGCGCAGAGTTCGTCGATCAGCTTCGTCGAGCAGCTTATTCTCAGCAAGAAAATGAGCTCGCTGGAGG
TGGCGACGTTCGCTTCACAGGTGTTCGGCTTTCCCTTGCTGGACCTTGCCGCCATCGAGCCGGAGCAACTGCCGCAGGGG
CTGGTGGACGAGGACCTGATCAGCTCGCGCCGCCTGTTGCCCTTGTACAAGCGGGGCAGCCGGCTGTTCATCGGCACCTC
CGACCCCACCCAGACCTCGGCCTTCCACGCCATTACCTTCAAGACTAGCCTGTCGGTCGAAGTCGTGGTGGTGGAGGACG
ACAAGCTCGGCAAGCTGATCGGCAGCTACCAGGCGGGTGGGGCCGAAGCGATCAAGGCCCTGGTGGACGAGGATTTTGGC
GACCTTGAGTTTGCCGAGCCGGATGCCGAGCCGGAAAAGACCGTCGCCCAGACCGAAGTGGACGATGCCCCCGTGGTCAA
GTTCATCCACAAGGTGCTGCTGGATGCGATCAACGGCGGGGCATCCGACGTCCATTTCGAGCCGTATGAGAAGTATTACC
GCATCCGCTACCGGGTGGATGGCGTGCTCAAGGAAGTCGCCCAGCCGCCCCTCTTGCTGAAGGAAAAGATTGCTTCACGC
ATCAAGGTGATCTCGCGGCTCGACATTTCGGAGAAGCGCGTGCCGCAGGACGGCCGCTTGAAGCTGGTGTTGTCCAAGAC
GCGTGCCATCGATTTCCGGGTCAGCACTCTGCCGACGCTGTTTGGCGAGAAGATCGTGATGCGTATTCTCGACCAATCGG
CGGCCACGCTGAATATCGAGCAGCTGGGGTTCGAGCCGGAACAGAAGGCGATGCTGCTCGAGGCCATCAGCCGCCCCTAT
GGCATGGTGCTGGTGACCGGTCCGACCGGCAGCGGCAAGACGGTGTCGCTCTATACCTGCCTGAACATCTTGAACAAGCC
GGACACCAATATTTCCACGGCGGAGGACCCGGTCGAAATCAACCTGCCGGGGATCAACCAGGTGAACGTCAACGAGCGCG
CGGGGCTGACCTTTTCGTCGGCGCTGAAAGCCTTTTTGCGCCAGGATCCGGACGTGATCATGGTGGGTGAAATCCGCGAT
TTTGAAACCGCCGACATCGCCATCAAGGCGGCACAGACCGGCCACATGGTCTTTTCCACGCTGCATACCAATAATGCTCC
CGCCACGCTAACGCGCTTGCTGAACATGGGGGTAGCGCCGTTCAACGTCGCCAGTTCCGTGCTGCTGATCATGGCGCAGC
GCCTGGCCAGACGCTTGTGTAACAATTGCAAGAAGCCGATGGATATTCCGAAAGAGGCGCTGTTACGGGCGGGCTACCGT
GAGGTGGAGCTGGACGGCAGTTGGCAGCCTTACGGACCCGTCGGGTGCCGCGAGTGTCGCGATTCGGGCTACCGGGGGCG
GGTGGGGATTTATGAGGTCATGCCCATCACCGATGCCATGACTCGTCTTATCATGAAAAACGGCACAGCGGTGGACATCG
CGGATCTGGCTCGGCAGGAGGGGATGGTCGATCTGCGTCGTGCCGGCTTGCTCAAGGTGAAGCAGGGCATCACCTCGCTG
GCGGAAATCGAAGCCGCCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2IZF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.957

100

0.555
  pilF Neisseria gonorrhoeae MS11

55.477

99.473

0.552
  pilB Acinetobacter baylyi ADP1

54.433

99.121

0.54
  pilB Legionella pneumophila strain ERS1305867

53.357

99.473

0.531
  pilB Vibrio campbellii strain DS40M4

47.703

99.473

0.475
  pilB Vibrio cholerae strain A1552

48.816

96.485

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

47.415

98.594

0.467
  pilF Thermus thermophilus HB27

38.927

100

0.395

Multiple sequence alignment