Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynTAK9802_RS00095 Genome accession   NZ_CP047937
Coordinates   21575..22651 (-) Length   358 a.a.
NCBI ID   WP_186496608.1    Uniprot ID   -
Organism   Synechococcus sp. TAK9802     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16575..27651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynTAK9802_RS00070 (SynTAK9802_00014) - 16998..17528 (+) 531 WP_186496600.1 RNA-binding protein -
  SynTAK9802_RS00075 (SynTAK9802_00015) dusA 17542..18537 (-) 996 WP_186496602.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynTAK9802_RS00080 (SynTAK9802_00016) msrB 18578..19081 (+) 504 WP_115131434.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynTAK9802_RS00085 (SynTAK9802_00017) - 19053..20321 (+) 1269 WP_186496604.1 NAD(P)/FAD-dependent oxidoreductase -
  SynTAK9802_RS00090 (SynTAK9802_00018) - 20296..21555 (-) 1260 WP_186496606.1 type II secretion system F family protein -
  SynTAK9802_RS00095 (SynTAK9802_00019) pilT 21575..22651 (-) 1077 WP_186496608.1 type IV pilus twitching motility protein PilT Machinery gene
  SynTAK9802_RS00100 (SynTAK9802_00020) - 22662..24524 (-) 1863 WP_186496610.1 GspE/PulE family protein -
  SynTAK9802_RS00105 (SynTAK9802_00021) grpE 24615..25292 (+) 678 WP_186496612.1 nucleotide exchange factor GrpE -
  SynTAK9802_RS00110 (SynTAK9802_00022) dnaJ 25292..26428 (+) 1137 WP_186496614.1 molecular chaperone DnaJ -
  SynTAK9802_RS00115 (SynTAK9802_00023) - 26425..26652 (+) 228 WP_186496616.1 sulfurtransferase TusA family protein -
  SynTAK9802_RS00120 (SynTAK9802_00024) rsgA 26678..27559 (+) 882 WP_186497896.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39261.72 Da        Isoelectric Point: 5.4454

>NTDB_id=418550 SynTAK9802_RS00095 WP_186496608.1 21575..22651(-) (pilT) [Synechococcus sp. TAK9802]
MDLMIEDLMEQLVQGGGSDLHLATGQPPYGRFSGELRPMTDRPLAEEDCNKLIFSMLNNSQRKTLEQTWELDCAYGLKGI
ARFRVNVYRQKGSYAACLRALGSTIPSVELLNLPPVVLETSARPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYQSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAVSAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVAVFSQTLCRRQNPAEGQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGEQGM
QTLEKALANLVLNGDVSRAEAMGKASKPSELQRLIGEI

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418550 SynTAK9802_RS00095 WP_186496608.1 21575..22651(-) (pilT) [Synechococcus sp. TAK9802]
ATGGATCTGATGATCGAAGACCTGATGGAACAGCTGGTGCAGGGCGGTGGCAGTGATCTGCACCTCGCCACCGGTCAACC
GCCCTATGGCCGGTTCAGCGGTGAACTGCGTCCGATGACGGACCGACCCCTGGCCGAGGAGGACTGCAACAAGTTGATCT
TCTCGATGCTGAACAACAGCCAGCGCAAGACCCTGGAGCAGACCTGGGAACTGGACTGCGCCTATGGCCTGAAAGGCATT
GCACGCTTCCGCGTCAACGTCTATCGCCAGAAAGGCAGCTATGCCGCCTGCCTGCGGGCCCTTGGCAGCACGATTCCCAG
CGTTGAACTGCTGAACCTGCCACCTGTGGTGCTGGAGACCAGCGCGCGGCCCCGGGGGCTTGTGCTGGTGACCGGTCCGA
CCGGATCAGGAAAAACCACCACCCTGGCGGCGCTGCTGGATCACATCAATCACACCCGCAGCGAACACATCCTCACGATC
GAAGACCCGATCGAATTCGTGTACCAGAGCGACAAAAGCCTGGTGCACCAACGCCAACTGAACGAAGACACCCGCAGCTT
CGCCAACGCCCTGCGCGCCGCCCTGCGGGAGGACCCTGACGTGATCCTGGTCGGGGAGATGCGGGATCTGGAGACCATTC
AATTGGCGGTGAGTGCCGCCGAAACCGGACATCTCGTCTTCGGAACCCTGCACACCAGCTCCGCCGCCCAGACCGTGGAC
CGCATGGTCGACGTCTTCCCACCGGAACAACAGACGCAGATCCGTGTGCAGCTCTCGGGCAGTTTGGTCGCTGTGTTTTC
CCAGACCCTCTGCCGGCGGCAGAACCCGGCAGAAGGACAGTTCGGTCGGGTGATGGCCCAGGAGATCCTGATCAACACAC
CCGCCACCGCCAACCTGATCCGCGAAGGAAAAACAGCCCAGCTCTATTCCCAAATCCAGACGGGGGGAGAACAGGGCATG
CAGACCCTGGAGAAAGCCTTGGCCAACCTTGTGCTCAACGGGGATGTGTCCCGCGCTGAAGCGATGGGCAAAGCCAGCAA
ACCAAGCGAATTGCAGCGCTTGATCGGTGAAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.314

96.927

0.517
  pilT Pseudomonas stutzeri DSM 10701

52.45

96.927

0.508
  pilT Legionella pneumophila strain Lp02

52.819

94.134

0.497
  pilT Legionella pneumophila strain ERS1305867

52.819

94.134

0.497
  pilT Acinetobacter baumannii D1279779

51.009

96.927

0.494
  pilT Acinetobacter baumannii strain A118

51.009

96.927

0.494
  pilT Acinetobacter nosocomialis M2

51.009

96.927

0.494
  pilT Neisseria meningitidis 8013

50.862

97.207

0.494
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.571

97.765

0.494
  pilT Neisseria gonorrhoeae MS11

50.575

97.207

0.492
  pilT Acinetobacter baylyi ADP1

50.432

96.927

0.489
  pilT Vibrio cholerae strain A1552

50

94.972

0.475
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.972

0.475
  pilU Pseudomonas stutzeri DSM 10701

38.746

98.045

0.38
  pilU Vibrio cholerae strain A1552

38.348

94.693

0.363

Multiple sequence alignment