Detailed information NTG0412406

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GTF75_RS01280
• Genome accession: NZ_CP047301
• Coordinates: 271337..272353 (+)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae O1 biovar El Tor strain DRC-193A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 266337..277353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTF75_RS01265 (GTF75_01265)	-	266879..267610 (-)	732	WP_000495223.1	glutathione peroxidase	-
GTF75_RS01270 (GTF75_01270)	oxyR	267750..268643 (+)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
GTF75_RS01275 (GTF75_01275)	-	268717..271194 (-)	2478	WP_000668733.1	penicillin-binding protein 1A	-
GTF75_RS01280 (GTF75_01280)	pilM	271337..272353 (+)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
GTF75_RS01285 (GTF75_01285)	pilN	272343..272927 (+)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
GTF75_RS01290 (GTF75_01290)	pilO	272920..273510 (+)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
GTF75_RS01295 (GTF75_01295)	pilP	273500..274018 (+)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
GTF75_RS01300 (GTF75_01300)	pilQ	274044..275759 (+)	1716	WP_000788426.1	type IV pilus secretin PilQ family protein	Machinery gene
GTF75_RS01305 (GTF75_01305)	aroK	275946..276470 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=412406 GTF75_RS01280 WP_000520444.1 271337..272353(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain DRC-193A]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=412406 GTF75_RS01280 WP_000520444.1 271337..272353(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain DRC-193A]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485