Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H4J01_RS03940 Genome accession   NZ_CP059840
Coordinates   801114..801923 (+) Length   269 a.a.
NCBI ID   WP_001340009.1    Uniprot ID   B7UI40
Organism   Escherichia coli strain E2348/69     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 796114..806923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4J01_RS03935 sslE 796345..800919 (+) 4575 WP_001340008.1 lipoprotein metalloprotease SslE -
  H4J01_RS03940 pilD 801114..801923 (+) 810 WP_001340009.1 prepilin peptidase PppA Machinery gene
  H4J01_RS03945 gspS2 801989..802399 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  H4J01_RS03950 gspC 802417..803376 (+) 960 WP_000135109.1 type II secretion system protein GspC -
  H4J01_RS03955 gspD 803406..805466 (+) 2061 WP_000498844.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29401.01 Da        Isoelectric Point: 8.3801

>NTDB_id=411282 H4J01_RS03940 WP_001340009.1 801114..801923(+) (pilD) [Escherichia coli strain E2348/69]
MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGETPSTQLKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=411282 H4J01_RS03940 WP_001340009.1 801114..801923(+) (pilD) [Escherichia coli strain E2348/69]
ATGCTTTTTGATGTTTTTCAGCAATATCCTGCTGCGATGCCTGTCCTGGCAACCGTCGGAGGATTGATTATCGGCAGTTT
TTTGAATGTGGTGATCTGGCGTTACCCTATCATGCTTCGCCAACAAATGGCGGAGTTTCACGGTGAAACGCCGAGCACGC
AGCTAAAAATAAGCCTGGCGCTGCCACGCTCGCATTGTCCGCATTGTCAGCAGACCATCCGCGTTCGTGACAACATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAATT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGACTGGCGGTGATGATATTATCCG
CGTGGCTGATTGCCGCGAGCGTCATTGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTAATTGCGGCATGGGCGCAGCAGAGTCCGCTCACGCTACAAGACGCAGTCACCGGCGTACTGGTGGGATTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCCG
CTTTAGGTGGCTGGGTGGGGGCGTTATCACTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UI40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.067

99.257

0.457
  pilD Vibrio campbellii strain DS40M4

41.758

100

0.424
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter nosocomialis M2

37.736

98.513

0.372
  pilD Acinetobacter baumannii D1279779

37.358

98.513

0.368