Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HR073_RS17685 Genome accession   NZ_CP054227
Coordinates   3759950..3761443 (-) Length   497 a.a.
NCBI ID   WP_000249334.1    Uniprot ID   -
Organism   Escherichia coli strain EcPF15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3754950..3766443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR073_RS17655 (HR073_17645) gspK 3755744..3756721 (-) 978 WP_000633218.1 type II secretion system minor pseudopilin GspK -
  HR073_RS17660 (HR073_17650) gspJ 3756718..3757323 (-) 606 WP_001250461.1 type II secretion system minor pseudopilin GspJ -
  HR073_RS17665 (HR073_17655) gspI 3757320..3757691 (-) 372 WP_000820109.1 type II secretion system minor pseudopilin GspI -
  HR073_RS17670 (HR073_17660) gspH 3757688..3758251 (-) 564 WP_001588244.1 type II secretion system minor pseudopilin GspH -
  HR073_RS17675 (HR073_17665) gspG 3758255..3758710 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  HR073_RS17680 (HR073_17670) gspF 3758727..3759950 (-) 1224 WP_001173436.1 type II secretion system inner membrane protein GspF -
  HR073_RS17685 (HR073_17675) pilB/pilB1 3759950..3761443 (-) 1494 WP_000249334.1 type II secretion system ATPase GspE Machinery gene
  HR073_RS17690 (HR073_17680) gspD 3761443..3763503 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  HR073_RS17695 (HR073_17685) gspC 3763533..3764492 (-) 960 WP_001366858.1 type II secretion system protein GspC -
  HR073_RS17700 (HR073_17690) gspS2 3764510..3764920 (-) 411 WP_001305972.1 type II secretion system pilot lipoprotein GspS-beta -
  HR073_RS17705 (HR073_17695) pilD 3764986..3765795 (-) 810 WP_000464638.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55181.28 Da        Isoelectric Point: 5.8815

>NTDB_id=398347 HR073_RS17685 WP_000249334.1 3759950..3761443(-) (pilB/pilB1) [Escherichia coli strain EcPF15]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDAHAVTLYYPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=398347 HR073_RS17685 WP_000249334.1 3759950..3761443(-) (pilB/pilB1) [Escherichia coli strain EcPF15]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGACTGAATGCCGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTCTTTACCCTCGCAGAAGAGCTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACTCTGTACTATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACCGCTATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAATGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment