Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BSN5_RS13565 Genome accession   NC_014976
Coordinates   2586630..2587079 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis BSn5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2581630..2592079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSN5_RS13530 (BSn5_13270) yckA 2581903..2582583 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  BSN5_RS13535 (BSn5_13275) yckB 2582593..2583456 (-) 864 WP_015715225.1 transporter substrate-binding domain-containing protein -
  BSN5_RS22010 - 2583618..2583813 (+) 196 Protein_2627 hypothetical protein -
  BSN5_RS13545 (BSn5_13280) - 2583853..2584167 (+) 315 Protein_2628 RDD family protein -
  BSN5_RS13550 (BSn5_13285) yckD 2584249..2584581 (+) 333 WP_015252849.1 YckD family protein -
  BSN5_RS13555 (BSn5_13290) bglC 2584735..2586168 (+) 1434 WP_003234614.1 6-phospho-beta-glucosidase -
  BSN5_RS13560 (BSn5_13295) nin/comJ 2586205..2586603 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  BSN5_RS13565 (BSn5_13300) nucA/comI 2586630..2587079 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  BSN5_RS13570 (BSn5_13305) tlpC 2587247..2588968 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  BSN5_RS13575 (BSn5_13310) hxlB 2589079..2589636 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  BSN5_RS13580 (BSn5_13315) hxlA 2589642..2590274 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  BSN5_RS13585 (BSn5_13320) hxlR 2590507..2590869 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=39783 BSN5_RS13565 WP_003234608.1 2586630..2587079(-) (nucA/comI) [Bacillus subtilis BSn5]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=39783 BSN5_RS13565 WP_003234608.1 2586630..2587079(-) (nucA/comI) [Bacillus subtilis BSn5]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment