Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GH769_RS00810 Genome accession   NZ_CP045767
Coordinates   163224..164432 (-) Length   402 a.a.
NCBI ID   WP_032903994.1    Uniprot ID   -
Organism   Pseudomonas sp. CFSAN084952     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 158224..169432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH769_RS00770 (GH769_00770) - 158232..159530 (-) 1299 WP_032903990.1 NAD(P)/FAD-dependent oxidoreductase -
  GH769_RS00775 (GH769_00775) - 159591..159764 (-) 174 WP_014716869.1 DUF3094 family protein -
  GH769_RS00780 (GH769_00780) - 159819..160448 (-) 630 WP_003188431.1 DUF1780 domain-containing protein -
  GH769_RS00785 (GH769_00785) - 160578..161267 (+) 690 WP_032903991.1 energy-coupling factor ABC transporter permease -
  GH769_RS00790 (GH769_00790) - 161332..161547 (+) 216 WP_003171680.1 hypothetical protein -
  GH769_RS00795 (GH769_00795) yacG 161544..161744 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  GH769_RS00800 (GH769_00800) coaE 161741..162364 (-) 624 WP_032903992.1 dephospho-CoA kinase -
  GH769_RS00805 (GH769_00805) pilD 162361..163227 (-) 867 WP_032903993.1 A24 family peptidase Machinery gene
  GH769_RS00810 (GH769_00810) pilC 163224..164432 (-) 1209 WP_032903994.1 type II secretion system F family protein Machinery gene
  GH769_RS00815 (GH769_00815) - 164634..165047 (+) 414 WP_014716874.1 pilin -
  GH769_RS00825 (GH769_00825) - 165337..165642 (+) 306 WP_014716875.1 DUF6388 family protein -
  GH769_RS00830 (GH769_00830) nadC 165692..166540 (-) 849 WP_014716876.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GH769_RS00835 (GH769_00835) - 166786..168933 (+) 2148 WP_153649471.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43778.41 Da        Isoelectric Point: 10.2753

>NTDB_id=396938 GH769_RS00810 WP_032903994.1 163224..164432(-) (pilC) [Pseudomonas sp. CFSAN084952]
MNDTSTIYAWEGINRKGRRVSGQIAGHNLALIKLQLRQQGICPERVRKKSYGLHIFKTRITPADIALFTRQLATLLKAGI
PLLQAFDIISEGVENPQLRELVKALKQHIASGHPLAAALRQYPRHFDELYCNLVAAGEMAGALETLLERVAIHLEKSQRL
KTRIKKAMTYPIAVLVIATGVSTVLLIHVVPQFQNLFAGVNGQLPGFTLGVIALSELLQQAWWMLGLGVGAALVVGRHAY
RTYPGFRHWWAAGLLKVPMAGKLLHKSAVARYARTLSTTFAAGVPLVQALGSVAGAVGNGPFKQAIERMRHDVSTGMQLN
QSMHNSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYESDVDTLVDNLTSLMEPLIMVVLGGIVGALVVAMYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=396938 GH769_RS00810 WP_032903994.1 163224..164432(-) (pilC) [Pseudomonas sp. CFSAN084952]
ATGAACGACACGTCGACAATTTATGCCTGGGAAGGCATCAACCGCAAAGGGCGCCGGGTGTCCGGGCAAATCGCCGGCCA
TAACCTTGCGCTGATCAAGCTACAACTGCGCCAGCAAGGGATCTGCCCTGAGCGTGTGCGCAAGAAATCTTATGGTTTAC
ACATCTTCAAGACGCGAATAACGCCTGCAGATATCGCCCTGTTCACTCGCCAGTTGGCCACCTTGCTCAAAGCCGGCATC
CCGCTGCTGCAAGCCTTCGACATCATCAGCGAAGGCGTCGAAAACCCACAGCTGCGCGAACTGGTCAAGGCGTTGAAACA
GCATATTGCCAGCGGCCACCCGTTGGCCGCTGCCCTGCGTCAATATCCCCGCCATTTCGATGAGCTGTACTGCAATCTGG
TGGCGGCTGGCGAAATGGCCGGCGCCCTGGAAACCCTGCTGGAGCGCGTGGCGATTCATTTGGAAAAAAGCCAACGGCTC
AAAACCAGGATCAAGAAAGCCATGACCTACCCTATCGCGGTACTGGTAATTGCCACAGGGGTGAGCACAGTGCTGTTGAT
CCATGTGGTGCCGCAATTCCAGAACTTGTTCGCCGGGGTCAACGGCCAATTGCCCGGGTTTACCCTGGGGGTCATCGCGC
TGTCGGAACTGCTGCAACAGGCGTGGTGGATGCTGGGGCTGGGTGTGGGCGCGGCGCTGGTTGTGGGGCGCCACGCCTAC
CGTACTTACCCGGGGTTTCGCCATTGGTGGGCAGCAGGTTTGTTGAAAGTGCCCATGGCAGGCAAACTGCTGCACAAATC
TGCGGTCGCCCGCTACGCTCGTACACTTTCCACCACCTTTGCGGCGGGCGTCCCTCTGGTACAGGCATTGGGTTCGGTGG
CCGGCGCCGTCGGCAATGGCCCCTTCAAACAGGCAATCGAACGTATGCGCCACGATGTATCCACAGGCATGCAGTTGAAT
CAATCGATGCATAACAGCGGCCTGTTCCCAGGCATGGCGATCCAGATGACCGCAATCGGGGAAGAGTCCGGCACCCTGGA
CCGCATGCTGGAAAAAGTCGCGAACCACTACGAGTCAGACGTGGATACCTTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTGATCATGGTCGTATTGGGGGGGATTGTCGGCGCGCTGGTAGTGGCCATGTACCTGCCGGTGTTCCAATTGGGGACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.538

99.005

0.57
  pilC Acinetobacter baylyi ADP1

50.505

98.507

0.498
  pilC Acinetobacter baumannii D1279779

50.127

98.259

0.493
  pilC Legionella pneumophila strain ERS1305867

48.354

98.259

0.475
  pilC Vibrio cholerae strain A1552

40.204

97.761

0.393
  pilG Neisseria gonorrhoeae MS11

40.104

95.522

0.383
  pilC Thermus thermophilus HB27

38.155

99.751

0.381
  pilG Neisseria meningitidis 44/76-A

39.583

95.522

0.378
  pilC Vibrio campbellii strain DS40M4

38.265

97.512

0.373

Multiple sequence alignment