Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GFH33_RS22720 Genome accession   NZ_CP045585
Coordinates   4394314..4395357 (-) Length   347 a.a.
NCBI ID   WP_001013180.1    Uniprot ID   A0A9X5WLU9
Organism   Bacillus thuringiensis strain x023     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4389314..4400357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH33_RS22675 (GFH33_22675) - 4389745..4389945 (-) 201 WP_000106083.1 YqzE family protein -
  GFH33_RS22680 (GFH33_22680) - 4389984..4390481 (-) 498 WP_000836650.1 shikimate kinase -
  GFH33_RS22685 (GFH33_22685) - 4390597..4391247 (-) 651 WP_000183862.1 2OG-Fe(II) oxygenase -
  GFH33_RS22690 (GFH33_22690) comGG 4391424..4391795 (-) 372 WP_001231273.1 competence type IV pilus minor pilin ComGG -
  GFH33_RS22695 (GFH33_22695) comGF 4391792..4392262 (-) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  GFH33_RS22700 (GFH33_22700) - 4392232..4392534 (-) 303 WP_000829473.1 hypothetical protein -
  GFH33_RS22705 (GFH33_22705) comGD 4392527..4392982 (-) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  GFH33_RS22710 (GFH33_22710) comGC 4392979..4393278 (-) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  GFH33_RS22715 (GFH33_22715) comGB 4393290..4394321 (-) 1032 WP_000475193.1 competence type IV pilus assembly protein ComGB -
  GFH33_RS22720 (GFH33_22720) comGA 4394314..4395357 (-) 1044 WP_001013180.1 competence type IV pilus ATPase ComGA Machinery gene
  GFH33_RS22725 (GFH33_22725) - 4395563..4396258 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  GFH33_RS22730 (GFH33_22730) - 4396384..4396626 (+) 243 WP_000440721.1 DUF2626 domain-containing protein -
  GFH33_RS22735 (GFH33_22735) - 4396733..4398127 (+) 1395 WP_001094330.1 L-cystine transporter -
  GFH33_RS22740 (GFH33_22740) - 4398207..4398599 (-) 393 WP_000875388.1 hypothetical protein -
  GFH33_RS22745 (GFH33_22745) - 4398687..4398902 (-) 216 WP_001008326.1 DUF3912 family protein -
  GFH33_RS22750 (GFH33_22750) - 4399155..4399466 (+) 312 WP_001093237.1 hypothetical protein -
  GFH33_RS22755 (GFH33_22755) - 4399501..4399989 (-) 489 WP_000764261.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39157.52 Da        Isoelectric Point: 8.5482

>NTDB_id=395340 GFH33_RS22720 WP_001013180.1 4394314..4395357(-) (comGA) [Bacillus thuringiensis strain x023]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGHELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=395340 GFH33_RS22720 WP_001013180.1 4394314..4395357(-) (comGA) [Bacillus thuringiensis strain x023]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCAGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTCGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGTTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGACATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559

Multiple sequence alignment