Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HKW69_RS05620 Genome accession   NZ_CP053080
Coordinates   1140588..1141568 (+) Length   326 a.a.
NCBI ID   WP_023152180.1    Uniprot ID   -
Organism   Escherichia coli strain HB37     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1135588..1146568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW69_RS05590 (HKW69_05600) yggM 1136193..1137200 (+) 1008 WP_000745230.1 DUF1202 family protein -
  HKW69_RS05595 (HKW69_05605) hemW 1137265..1138401 (-) 1137 WP_000239984.1 radical SAM family heme chaperone HemW -
  HKW69_RS05600 (HKW69_05610) rdgB 1138394..1138987 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  HKW69_RS05605 (HKW69_05615) yggU 1138995..1139285 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  HKW69_RS05610 (HKW69_05620) yggT 1139282..1139848 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  HKW69_RS05615 (HKW69_05625) yggS 1139866..1140570 (-) 705 WP_023152179.1 pyridoxal phosphate homeostasis protein -
  HKW69_RS05620 (HKW69_05630) pilT 1140588..1141568 (+) 981 WP_023152180.1 type IV pilus twitching motility protein PilT Machinery gene
  HKW69_RS05625 (HKW69_05635) ruvX 1141742..1142158 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  HKW69_RS05630 (HKW69_05640) yqgE 1142158..1142721 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  HKW69_RS05635 (HKW69_05645) gshB 1142830..1143780 (-) 951 WP_000593259.1 glutathione synthase -
  HKW69_RS05640 (HKW69_05650) rsmE 1143793..1144524 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HKW69_RS05645 (HKW69_05655) endA 1144604..1145311 (-) 708 WP_001305312.1 deoxyribonuclease I -
  HKW69_RS05650 (HKW69_05660) yggI 1145406..1145903 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36004.21 Da        Isoelectric Point: 5.7980

>NTDB_id=389710 HKW69_RS05620 WP_023152180.1 1140588..1141568(+) (pilT) [Escherichia coli strain HB37]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDMILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=389710 HKW69_RS05620 WP_023152180.1 1140588..1141568(+) (pilT) [Escherichia coli strain HB37]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATATGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

41.009

97.239

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment