Detailed information NTG0388227

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: HIR76_RS01665
• Genome accession: NZ_CP051860
• Coordinates: 304438..305559 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 299438..310559

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HIR76_RS01640 (HIR76_03860)	ycdA	299446..300510 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
HIR76_RS01645 (HIR76_03855)	-	300533..300664 (-)	132	WP_003246334.1	hypothetical protein	-
HIR76_RS01650 (HIR76_03850)	ycdB	300838..302256 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
HIR76_RS01655 (HIR76_03845)	ycdC	302443..303804 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
HIR76_RS01660 (HIR76_03840)	cwlK	303812..304315 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
HIR76_RS01665 (HIR76_03835)	rapF	304438..305559 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
HIR76_RS01670 (HIR76_03830)	ycdF	305666..306442 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
HIR76_RS01675 (HIR76_03825)	ycdG	306467..308152 (+)	1686	WP_003234736.1	alpha-glucosidase	-
HIR76_RS01680 (HIR76_03820)	znuA	308340..309299 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
HIR76_RS01685 (HIR76_03815)	znuC	309355..310050 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=388227 HIR76_RS01665 WP_003246478.1 304438..305559(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=388227 HIR76_RS01665 WP_003246478.1 304438..305559(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405