Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   F0362_RS00540 Genome accession   NZ_CP043830
Coordinates   97672..99048 (+) Length   458 a.a.
NCBI ID   WP_001085199.1    Uniprot ID   A0A9W5QZX4
Organism   Bacillus sp. BS98     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92672..104048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0362_RS00520 (F0362_00520) ctsR 92869..93330 (+) 462 WP_001244560.1 transcriptional regulator CtsR -
  F0362_RS00525 (F0362_00525) - 93501..94049 (+) 549 WP_000128388.1 UvrB/UvrC motif-containing protein -
  F0362_RS00530 (F0362_00530) - 94054..95118 (+) 1065 WP_000050835.1 protein arginine kinase -
  F0362_RS00535 (F0362_00535) clpC 95141..97576 (+) 2436 WP_000971171.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  F0362_RS00540 (F0362_00540) radA 97672..99048 (+) 1377 WP_001085199.1 DNA repair protein RadA Machinery gene
  F0362_RS00545 (F0362_00545) disA 99052..100125 (+) 1074 WP_000392164.1 DNA integrity scanning diadenylate cyclase DisA -
  F0362_RS00550 (F0362_00550) - 100286..101389 (+) 1104 WP_000919680.1 PIN/TRAM domain-containing protein -
  F0362_RS00555 (F0362_00555) ispD 101406..102086 (+) 681 WP_000288308.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  F0362_RS00560 (F0362_00560) ispF 102203..102679 (+) 477 WP_080326879.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49899.60 Da        Isoelectric Point: 6.9053

>NTDB_id=386052 F0362_RS00540 WP_001085199.1 97672..99048(+) (radA) [Bacillus sp. BS98]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETEFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRVIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=386052 F0362_RS00540 WP_001085199.1 97672..99048(+) (radA) [Bacillus sp. BS98]
ATGGCTAAAAAGAAAACGAAATTTATATGTCAAGAATGTGGTTATCAGTCACCGAAATATATGGGTAAATGTCCCGGATG
TGGTCAATGGAATACACTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGGCGCCTTAATTACGCGAATGCAATTCAAA
CGGAAGTAACAAAACCAAGACGTCTTACTGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACGGAATTTCAAGAGTTT
AACCGTGTGCTTGGTGGCGGAATTGTAGATGGGTCCTTAGTACTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
ACTATTACAGATTTCATCGCAATTAGCAGATTCTTCATATGATGTATTATACATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGCAATCTATTTGTTGTAGCAGAGACAGACTTGCAGCGTATTGCA
GCACATATTGAAGAAATGAATCCAGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
GCCAGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACAAAAGGAATCCCTATTTTCA
TCGTCGGGCATGTGACAAAAGAAGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTCTACTTT
GAGGGAGATCGACACCATACGTATCGTATTTTACGAGCTGTGAAGAACCGTTTTGGTTCCACGAATGAAATGGGTATCTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTTTTAAATCCTTCTGAAATCTTCCTTGAGGAAAGGCCGGTTGGTGTTGCAG
GGTCAACAGTAGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCTTTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTTTCGCTTATTATGGCAGTATTAGAAAAAAGAACAGG
CTTACTATTGCAAAATCAAGATGCATATTTAAAAGTAGCTGGTGGTTTGAAATTAGATGAACCGGCAATTGATTTAGCAG
TGGCTTTAAGTATAGCCTCAAGTTTTAGGGATAAATCTACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGTGTTATTATTCC
TAGAAAAAACTTAGGAGGATGGACAATTCCAGATGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus pneumoniae Rx1

64.238

98.908

0.635
  radA Streptococcus pneumoniae D39

64.238

98.908

0.635
  radA Streptococcus pneumoniae R6

64.238

98.908

0.635
  radA Streptococcus pneumoniae TIGR4

64.238

98.908

0.635
  radA Streptococcus mitis SK321

64.238

98.908

0.635
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633

Multiple sequence alignment