Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   SP670_RS10770 Genome accession   NC_014498
Coordinates   1988428..1988889 (-) Length   153 a.a.
NCBI ID   WP_000250523.1    Uniprot ID   A0A0B7M7T3
Organism   Streptococcus pneumoniae 670-6B     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1983428..1993889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP670_RS10735 (SP670_2121) - 1983740..1984564 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  SP670_RS10740 (SP670_2122) rnpA 1984539..1984910 (-) 372 WP_000739241.1 ribonuclease P protein component -
  SP670_RS10745 (SP670_2123) - 1985058..1986248 (-) 1191 WP_000167766.1 acetate kinase -
  SP670_RS10750 (SP670_2124) comYH 1986299..1987252 (-) 954 WP_000345085.1 class I SAM-dependent methyltransferase Machinery gene
  SP670_RS13660 - 1987313..1987900 (-) 588 Protein_2022 class I SAM-dependent methyltransferase -
  SP670_RS10765 (SP670_2127) comGG/cglG 1988037..1988450 (-) 414 WP_000265619.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP670_RS10770 (SP670_2128) comGF/cglF 1988428..1988889 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP670_RS10775 (SP670_2129) comGE/cglE 1988852..1989154 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP670_RS10780 (SP670_2130) comGD/cglD 1989117..1989521 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP670_RS10785 (SP670_2131) comGC/cglC 1989514..1989780 (-) 267 WP_000962026.1 competence type IV pilus major pilin ComGC Machinery gene
  SP670_RS10790 (SP670_2132) - 1989782..1990039 (-) 258 WP_000698513.1 hypothetical protein -
  SP670_RS10795 (SP670_2133) - 1990271..1991227 (-) 957 WP_000350474.1 N-acetylmuramoyl-L-alanine amidase family protein -
  SP670_RS10800 (SP670_2134) - 1991227..1991562 (-) 336 WP_001186218.1 phage holin -
  SP670_RS10805 (SP670_2135) - 1991566..1991865 (-) 300 WP_001811580.1 hypothetical protein -
  SP670_RS10810 (SP670_2136) - 1991874..1992224 (-) 351 WP_000852245.1 hypothetical protein -
  SP670_RS10815 (SP670_2137) - 1992227..1992430 (-) 204 WP_001091115.1 hypothetical protein -
  SP670_RS14675 (SP670_2138) - 1992411..1992527 (-) 117 WP_001063633.1 hypothetical protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17821.32 Da        Isoelectric Point: 6.4832

>NTDB_id=38325 SP670_RS10770 WP_000250523.1 1988428..1988889(-) (comGF/cglF) [Streptococcus pneumoniae 670-6B]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=38325 SP670_RS10770 WP_000250523.1 1988428..1988889(-) (comGF/cglF) [Streptococcus pneumoniae 670-6B]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M7T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae R6

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae TIGR4

94.771

100

0.948
  comGF/cglF Streptococcus mitis SK321

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

92.157

100

0.922

Multiple sequence alignment