Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HCN55_RS13850 Genome accession   NZ_CP050532
Coordinates   2645689..2646099 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis str. SMY     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2640689..2651099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCN55_RS13820 (HCN55_13825) - 2640776..2640916 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  HCN55_RS13825 (HCN55_13830) yqeF 2641222..2641953 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  HCN55_RS13830 (HCN55_13835) cwlH 2642205..2642957 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  HCN55_RS13835 (HCN55_13840) yqeD 2643144..2643770 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  HCN55_RS13840 (HCN55_13845) gnd 2643789..2644682 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  HCN55_RS13845 (HCN55_13850) yqeB 2644934..2645656 (+) 723 WP_010886572.1 hypothetical protein -
  HCN55_RS13850 (HCN55_13855) nucA/comI 2645689..2646099 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  HCN55_RS13855 (HCN55_13860) - 2646295..2646642 (+) 348 Protein_2692 sigma-70 family RNA polymerase sigma factor -
  HCN55_RS13860 (HCN55_13865) spoIVCA 2646673..2648133 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  HCN55_RS22135 - 2648091..2648269 (-) 179 Protein_2694 hypothetical protein -
  HCN55_RS13865 (HCN55_13870) arsC 2648624..2649043 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  HCN55_RS13870 (HCN55_13875) acr3 2649055..2650095 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  HCN55_RS13875 (HCN55_13880) arsK 2650118..2650558 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  HCN55_RS13880 (HCN55_13885) arsR 2650619..2650936 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=381383 HCN55_RS13850 WP_009967785.1 2645689..2646099(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. SMY]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=381383 HCN55_RS13850 WP_009967785.1 2645689..2646099(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. SMY]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529