Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AOLE_RS17815 Genome accession   NC_014259
Coordinates   3791486..3793198 (+) Length   570 a.a.
NCBI ID   WP_023274331.1    Uniprot ID   -
Organism   Acinetobacter oleivorans DR1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3786486..3798198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOLE_RS17780 (AOLE_17755) nusA 3787372..3788856 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  AOLE_RS17785 (AOLE_17760) rimP 3788893..3789420 (-) 528 WP_004795010.1 ribosome maturation factor RimP -
  AOLE_RS17805 (AOLE_17770) secG 3790058..3790387 (-) 330 WP_004795012.1 preprotein translocase subunit SecG -
  AOLE_RS17810 (AOLE_17775) tpiA 3790400..3791194 (-) 795 WP_013199077.1 triose-phosphate isomerase -
  AOLE_RS17815 (AOLE_17785) pilB 3791486..3793198 (+) 1713 WP_023274331.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOLE_RS17820 (AOLE_17790) pilC 3793226..3794452 (+) 1227 WP_013199080.1 type II secretion system F family protein Machinery gene
  AOLE_RS20825 (AOLE_17795) pilD 3794452..3795312 (+) 861 WP_013199081.1 A24 family peptidase Machinery gene
  AOLE_RS17830 (AOLE_17800) coaE 3795314..3795910 (+) 597 WP_013199082.1 dephospho-CoA kinase -
  AOLE_RS17835 (AOLE_17805) - 3795907..3796821 (-) 915 WP_013199083.1 DMT family transporter -
  AOLE_RS17840 (AOLE_17810) rlmB 3796857..3797606 (-) 750 WP_004795029.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AOLE_RS17845 (AOLE_17815) - 3797709..3798035 (-) 327 WP_004795031.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63491.87 Da        Isoelectric Point: 4.9650

>NTDB_id=37698 AOLE_RS17815 WP_023274331.1 3791486..3793198(+) (pilB) [Acinetobacter oleivorans DR1]
MSVIHTSPKFSGFFRRLIEEKHVSAATMQAALDAAKRAKQDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLVQRGQILYVATSNPSNIDAIDAIRFNSKLMVEPVIVEHHKLEKILVQQFTEESNFEFSDEEF
DLDVDLDTSAAPEEDEDTPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKVPTDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=37698 AOLE_RS17815 WP_023274331.1 3791486..3793198(+) (pilB) [Acinetobacter oleivorans DR1]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAATCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAGCAGCATTAGATGCAGCAAAAAGAGCTAAACAGGATACTGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAGACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGATCAGAAACTGATTTTAAAACATCGGATCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGTAATCCCAGCAATATTGATGCTATTGATGCTATTCGCTTTAACAGCAAGCTTATGGTTGAGCCTGTTATTG
TCGAGCACCATAAACTCGAAAAAATTCTGGTACAGCAATTTACTGAAGAAAGTAATTTCGAGTTTAGTGATGAAGAATTT
GATCTAGATGTAGATCTTGATACCTCAGCTGCTCCAGAAGAGGATGAGGATACTCCACAAGGCGATGAAGCACCTATTGT
TAAATATATTAACAAATTATTAATTGATGCGATTCGTATGGGGGCTTCTGATTTACATTTCGAACCTTATGAAAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTTTTGCGTCAAATTGCAAATCCGCCTTTGCAGCTGGCCAATCGTTTGGCTTCA
CGCTTAAAAGTGATGTCTCAAATGGACATTTCTGAAAAGCGTGTCCCTCAAGATGGTCGTATCAAGCTCAAGCTATCAAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CGAGTGCTATGCTGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCGCTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATCACTGGTCCGACAGGTTCTGGTAAGACTGTATCTTTATATACAGGCCTCAATATCTTAAATAC
TGAAAGTTCTAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGCATTAATCAGGTTAACGTGAACCCTA
AGGTAGGTCTGACTTTTTCAGCTGCACTTAAGTCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAAGCGGCTCAGACAGGTCATATGGTGATGTCCACGCTACATACCAACAGTGC
GCCTGAAACACTGACTCGTCTACGCAATATGGGAGTTCCATCTTTCAATATTGCGACATCGGTCAATCTGGTCATTGCTC
AGCGTTTGGCACGTCGACTATGTTCTCAATGTAAAGTACCTACTGACATTCCTAAACAAAGCCTATTGGAAATGGGTTTT
ACCGAACAAGATCTGACACATCCCGATTTTCAAGTTTTTCAACCCGTCGGTTGTCCAGAATGTCGTGAGGGTTACAAAGG
TCGAGTTGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCCGCTTCTGAAAAACTGGGGTTTAATAATCTACGTCGCTCAGGTTTAAAGAAAGTTATGCAAGGTGTTACTTCT
TTACAAGAAGTCAATCGCGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.211

100

0.942
  pilB Acinetobacter baylyi ADP1

79.474

100

0.795
  pilB Legionella pneumophila strain ERS1305867

51.761

99.649

0.516
  pilF Neisseria gonorrhoeae MS11

48.932

98.596

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.901

92.281

0.479
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379

Multiple sequence alignment