Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FPL17_RS09640 Genome accession   NZ_CP041970
Coordinates   2121124..2122242 (+) Length   372 a.a.
NCBI ID   WP_033134346.1    Uniprot ID   -
Organism   Acinetobacter dispersus strain NCCP 16014     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2116124..2127242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPL17_RS09620 (FPL17_09750) - 2117593..2118260 (+) 668 Protein_1922 HAD family hydrolase -
  FPL17_RS09625 (FPL17_09755) - 2118321..2119061 (-) 741 WP_159884774.1 hypothetical protein -
  FPL17_RS09630 (FPL17_09760) - 2119250..2119936 (-) 687 WP_159884776.1 YggS family pyridoxal phosphate-dependent enzyme -
  FPL17_RS09635 (FPL17_09765) pilT 2120062..2121099 (+) 1038 WP_004655144.1 type IV pilus twitching motility protein PilT Machinery gene
  FPL17_RS09640 (FPL17_09770) pilU 2121124..2122242 (+) 1119 WP_033134346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FPL17_RS09645 (FPL17_09775) fur 2122339..2122776 (-) 438 WP_159884778.1 ferric iron uptake transcriptional regulator -
  FPL17_RS09650 (FPL17_09780) - 2122888..2123286 (+) 399 WP_004805189.1 outer membrane protein assembly factor BamE -
  FPL17_RS09655 (FPL17_09785) - 2123349..2123663 (-) 315 WP_159884780.1 RnfH family protein -
  FPL17_RS09660 (FPL17_09790) - 2123663..2124745 (-) 1083 WP_159884782.1 hypothetical protein -
  FPL17_RS09665 (FPL17_09800) - 2124990..2125445 (-) 456 WP_004655155.1 bacteriohemerythrin -
  FPL17_RS09670 (FPL17_09805) - 2125731..2126579 (-) 849 WP_026057514.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41708.88 Da        Isoelectric Point: 6.3492

>NTDB_id=375074 FPL17_RS09640 WP_033134346.1 2121124..2122242(+) (pilU) [Acinetobacter dispersus strain NCCP 16014]
MDFNDLLNLMIQQNASDLFVTADVEPSMKINGQIVPVSKSKLSGEIVGQLVQSIMTDKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRKIETHIPSMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGFRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=375074 FPL17_RS09640 WP_033134346.1 2121124..2122242(+) (pilU) [Acinetobacter dispersus strain NCCP 16014]
ATGGATTTTAATGATTTACTCAATTTGATGATTCAACAAAATGCATCGGACTTATTTGTCACTGCGGATGTTGAACCTTC
AATGAAAATTAACGGGCAGATCGTGCCTGTTTCCAAATCGAAACTTTCAGGGGAAATTGTGGGTCAACTGGTTCAATCCA
TTATGACCGACAAGCAGCGTAAAGAGTTTGCAGAGACGCGTGAATGTAACTTTGCGATCATGAACCGTGAAAAGACAGCG
CGTTTCCGTGTCAGTGCTTTTCAACAACGTGATATGCCAGGCATGGTATTGCGTAAGATCGAAACCCATATTCCAAGCAT
GGATGATCTAAAACTGCCAGAAGTGCTGAAAGAATTGGCGATGACCAAACGTGGCATCATCATCTTTGTAGGTGCAACAG
GTACCGGTAAATCGACTTCTTTGGCGTCAATGATTGGTTTCCGCAACCAGAATTCTAAAGGTCATATCATCACCATTGAA
GACCCGATCGAATTTGTGCATCAACATGCAGGCTGTATCGTGACGCAACGTGAAGTCGGGATTGATACTGATTCATTTGA
AGTGGCATTGAAAAATACGCTACGTCAGGCACCAGATGTGATCTTAATTGGTGAGATTCGTTCGCGTGAAACTATGGACT
ATGCGATTGCTTTCGCAGAAACTGGTCACTTAGTACTCGCGACCTTGCACGCCAACAATGCCAACCAAGCCTTAGACCGT
ATTATTCACTTCTTTGAAGCTGACCGTCATAGTCAGCTGTACATGGACTTGTCACTCAACTTAAAAGCACTCGTCGCACA
ACAATTGATTCCAACCCCAGACGGTAATTCACGTCGTGCTGCCATTGAGATTTTGATTAATACACCGCTTTTAGCTGACT
ATATCCGTAAGGGTGAAATTCATGAGATTAAAGATCTGATGAAACGTTCACGCGAATTGGGAATGCAAACCTTTGACCAA
GCCTTATTTGACCTTTATAAAGCAGGTCAGATTACCTATAAGGATGCATTGAAGCATGCAGACTCACCAAACGATCTACG
CCTCACCATCAAACTTTCCGAAGAAGGCGCAGACCAATTATTGAGTGCCAGTCGAAATATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

70.35

99.731

0.702
  pilU Vibrio cholerae strain A1552

54.054

99.462

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.618

93.011

0.387
  pilT Legionella pneumophila strain Lp02

41.071

90.323

0.371
  pilT Legionella pneumophila strain ERS1305867

41.071

90.323

0.371
  pilT Neisseria gonorrhoeae MS11

40

91.398

0.366
  pilT Neisseria meningitidis 8013

40

91.398

0.366
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36

Multiple sequence alignment