Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FOG18_RS10135 Genome accession   NZ_CP041668
Coordinates   2270518..2271582 (-) Length   354 a.a.
NCBI ID   WP_143868434.1    Uniprot ID   -
Organism   Legionella israelensis strain L18-01051     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2265518..2276582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOG18_RS10110 (FOG18_10110) aroK 2266057..2266578 (-) 522 WP_172460999.1 shikimate kinase AroK -
  FOG18_RS10115 (FOG18_10115) pilQ 2266657..2268798 (-) 2142 WP_143868428.1 type IV pilus secretin PilQ family protein Machinery gene
  FOG18_RS10120 (FOG18_10120) - 2268801..2269334 (-) 534 WP_143868430.1 pilus assembly protein PilP -
  FOG18_RS10125 (FOG18_10125) pilO 2269331..2269930 (-) 600 WP_143868432.1 type 4a pilus biogenesis protein PilO Machinery gene
  FOG18_RS10130 (FOG18_10130) pilN 2269942..2270514 (-) 573 WP_058501753.1 PilN domain-containing protein Machinery gene
  FOG18_RS10135 (FOG18_10135) pilM 2270518..2271582 (-) 1065 WP_143868434.1 type IV pilus assembly protein PilM Machinery gene
  FOG18_RS10140 (FOG18_10140) - 2271839..2272843 (+) 1005 WP_143868436.1 hypothetical protein -
  FOG18_RS10145 (FOG18_10145) - 2273034..2275397 (+) 2364 WP_143868438.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39004.07 Da        Isoelectric Point: 4.5440

>NTDB_id=372946 FOG18_RS10135 WP_143868434.1 2270518..2271582(-) (pilM) [Legionella israelensis strain L18-01051]
MLKLLKPKKKSILGIDVSSTSVKILEISGEAPNYQLNAYASELLPEKAVEANVIKDVDAVAGCIKRATSRAKFSTKLAAA
AVPDSAVINKVIQINEGLNDEEMEELVVMEADKYIPYPIDEINIDFEILGPSAKNSTMLDVLIVASRAENVSNRAEVLTQ
AGLEAKIVDVESYAVERAVQLIAPDLPAEGVDKVIAVIDVGANYTHLFVLHGMKTVFSREEEFGARLLIESLAQRYEMTY
EQALEAYMQNQLLEDYKETVLEPFQEMMLLQVKRTLQFFYSTSNHGFVDHILLAGGVARQEGLVDLIQQQLGVPTTIANP
FKHMQLGKMLNPEVLNKEAPALLVACGLALRSIE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=372946 FOG18_RS10135 WP_143868434.1 2270518..2271582(-) (pilM) [Legionella israelensis strain L18-01051]
GTGCTCAAATTGCTTAAGCCTAAAAAGAAATCAATATTGGGTATAGACGTCAGCTCAACTTCCGTTAAGATCCTGGAGAT
TTCTGGTGAGGCTCCGAATTATCAGTTGAATGCTTATGCCAGCGAACTTTTGCCCGAAAAAGCAGTAGAAGCGAATGTAA
TTAAGGATGTTGATGCGGTAGCTGGATGCATTAAAAGAGCAACTTCCCGCGCAAAATTTTCCACTAAACTGGCGGCTGCA
GCGGTACCTGACTCCGCTGTCATTAATAAGGTAATACAAATCAATGAAGGCTTAAATGACGAGGAGATGGAAGAACTGGT
CGTCATGGAAGCTGATAAATATATCCCTTACCCTATAGATGAAATTAATATTGATTTTGAAATCCTTGGGCCTTCAGCAA
AAAATTCCACCATGCTTGATGTGTTGATTGTCGCATCCCGAGCGGAAAATGTAAGTAATCGAGCTGAGGTACTTACCCAG
GCAGGTCTTGAGGCTAAAATAGTTGATGTTGAATCTTATGCTGTCGAACGGGCAGTACAGTTAATAGCCCCTGATCTTCC
GGCGGAAGGGGTAGATAAAGTTATTGCTGTGATTGATGTAGGAGCCAACTACACGCATTTATTTGTTTTGCATGGAATGA
AAACGGTGTTTTCCCGTGAAGAGGAATTTGGTGCCCGACTTCTCATTGAATCACTGGCACAGCGATATGAAATGACCTAC
GAACAGGCTCTGGAAGCCTACATGCAAAATCAGCTGCTCGAAGATTACAAGGAAACGGTACTGGAGCCTTTTCAGGAAAT
GATGCTGTTACAGGTTAAACGTACCTTGCAGTTTTTTTATTCTACCAGCAACCATGGTTTTGTGGATCATATTCTTCTTG
CAGGTGGAGTGGCACGGCAGGAAGGACTGGTTGATCTTATCCAGCAACAATTAGGAGTACCTACGACCATTGCAAATCCC
TTTAAACATATGCAATTGGGTAAAATGCTTAACCCGGAAGTCCTCAATAAGGAGGCCCCTGCATTGCTTGTTGCTTGTGG
ATTGGCACTGAGGTCAATAGAGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

66.384

100

0.664
  comM Acinetobacter baylyi ADP1

42.254

100

0.424
  pilM Acinetobacter baumannii D1279779

42.09

100

0.421
  comM Acinetobacter nosocomialis M2

42.09

100

0.421

Multiple sequence alignment