Detailed information NTG0372893

Bioinformatically predicted

Overview

• Name: pilB/pilB1
• Type: Machinery gene
• Locus tag: G5T40_RS01055
• Genome accession: NZ_CP049196
• Coordinates: 219336..220829 (+)
• Length: 497 a.a.
• NCBI ID: WP_000249312.1
• Uniprot ID: -
• Organism: Escherichia coli strain E118
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 214336..225829

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G5T40_RS01035	pilD	214984..215793 (+)	810	WP_164476935.1	prepilin peptidase PppA	Machinery gene
G5T40_RS01040	gspS2	215859..216269 (+)	411	WP_001300497.1	type II secretion system pilot lipoprotein GspS-beta	-
G5T40_RS01045	gspC	216287..217246 (+)	960	WP_000135079.1	type II secretion system protein GspC	-
G5T40_RS01050	gspD	217276..219336 (+)	2061	WP_000498829.1	type II secretion system secretin GspD	-
G5T40_RS01055	pilB/pilB1	219336..220829 (+)	1494	WP_000249312.1	type II secretion system ATPase GspE	Machinery gene
G5T40_RS01060	gspF	220829..222052 (+)	1224	WP_089502605.1	type II secretion system inner membrane protein GspF	-
G5T40_RS01065	gspG	222069..222524 (+)	456	WP_001087296.1	type II secretion system major pseudopilin GspG	-
G5T40_RS01070	gspH	222528..223091 (+)	564	WP_001115118.1	type II secretion system minor pseudopilin GspH	-
G5T40_RS01075	gspI	223088..223459 (+)	372	WP_000820140.1	type II secretion system minor pseudopilin GspI	-
G5T40_RS01080	gspJ	223456..224061 (+)	606	WP_021547736.1	type II secretion system minor pseudopilin GspJ	-
G5T40_RS01085	gspK	224058..225035 (+)	978	WP_000631648.1	type II secretion system minor pseudopilin GspK	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 55164.31 Da
• Isoelectric Point: 5.9081

>NTDB_id=372893 G5T40_RS01055 WP_000249312.1 219336..220829(+) (pilB/pilB1) [Escherichia coli strain E118]
MVPVAQETIANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYIIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide

• Length: 1494 bp

>NTDB_id=372893 G5T40_RS01055 WP_000249312.1 219336..220829(+) (pilB/pilB1) [Escherichia coli strain E118]
ATGGTGCCTGTAGCACAGGAAACCATCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACACTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATAATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTACGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (123-389)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB/pilB1	Synechocystis sp. PCC 6803	39.556	100	0.431
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	47.529	85.513	0.406
pilB	Legionella pneumophila strain ERS1305867	42.398	93.964	0.398
pilB	Vibrio parahaemolyticus RIMD 2210633	48.111	79.879	0.384
pilB	Vibrio campbellii strain DS40M4	47.222	79.678	0.376
pilB	Acinetobacter baumannii D1279779	46.366	80.282	0.372
pilB	Vibrio cholerae strain A1552	45.96	79.678	0.366
pilB	Acinetobacter baylyi ADP1	46.41	78.471	0.364
pilF	Thermus thermophilus HB27	45	80.483	0.362