Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FMO13_RS05330 Genome accession   NZ_CP041382
Coordinates   1275174..1276883 (-) Length   569 a.a.
NCBI ID   WP_057677884.1    Uniprot ID   -
Organism   Xanthomonas phaseoli pv. dieffenbachiae strain PL36     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1270174..1281883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FMO13_RS05310 (FMO13_05505) - 1271252..1272865 (+) 1614 WP_209225620.1 sensor histidine kinase -
  FMO13_RS05315 (FMO13_05510) pilR 1273127..1274581 (+) 1455 WP_017155148.1 sigma-54-dependent transcriptional regulator Regulator
  FMO13_RS05320 (FMO13_05515) - 1274785..1274946 (-) 162 Protein_1048 serine/threonine protein kinase -
  FMO13_RS05325 (FMO13_05520) - 1274993..1275112 (-) 120 WP_057687106.1 hypothetical protein -
  FMO13_RS05330 (FMO13_05525) pilB 1275174..1276883 (-) 1710 WP_057677884.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FMO13_RS05335 (FMO13_05530) pilA 1277041..1277469 (-) 429 WP_057677883.1 pilin Machinery gene
  FMO13_RS05340 (FMO13_05535) pilC 1277810..1279072 (+) 1263 WP_057677885.1 type II secretion system F family protein Machinery gene
  FMO13_RS05345 (FMO13_05540) - 1279079..1279942 (+) 864 WP_003491180.1 prepilin peptidase -
  FMO13_RS05350 (FMO13_05545) coaE 1279956..1280564 (+) 609 WP_022558665.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61685.58 Da        Isoelectric Point: 5.4503

>NTDB_id=371763 FMO13_RS05330 WP_057677884.1 1275174..1276883(-) (pilB) [Xanthomonas phaseoli pv. dieffenbachiae strain PL36]
MGITGIARRLVQDGALDEASARTAMDQAAAAKIPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDANQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAANGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=371763 FMO13_RS05330 WP_057677884.1 1275174..1276883(-) (pilB) [Xanthomonas phaseoli pv. dieffenbachiae strain PL36]
GTGGGAATTACAGGCATTGCACGCCGTTTGGTGCAGGATGGAGCACTGGATGAGGCAAGTGCCCGTACCGCAATGGACCA
AGCCGCGGCGGCGAAGATTCCGCTTCCCCAATGGTTCTCCGAGAAAAAGCTGGTTTCAGCGTCTCAATTGGCCGCTGCCA
ATGCAGTCGAGTTCGGCATGCCACTACTGGACGTGTCGGCATTCGACGCCAACCAAAACGCGGTGAAGCTGGTCAGCGAG
GAATTGCTCCAGAAGCATCAAGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCATTCTTGTCGACGAGGATCAGATCCGCC
GGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGCAACGACGACGAGGAGATGGGGGATCTG
GACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATTAGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGCTTGCGCATCGATGGCCTGCTGAAGAATGTGGCCAAGGCGCCGGTGAAGCTGAATCAGCGCATCGCAGCA
CGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGTGAGAAGGTGGTGCTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGTATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAGTTGTTCCTGGAAGCGATCCACAAGCCG
TACGGGATGGTACTGGTGACCGGGCCGACTGGCTCGGGCAAGACCGTGTCGCTGTACACGGCGCTGGGTATCCTCAATGA
CGAGACGCGCAATATCTCCACTGCGGAGGACCCGGTCGAAATCCGCTTGCCTGGTGTCAACCAGGTGCAGCAGAACAACA
AGCGTGGCATGACCTTCGCCGCAGCCTTGCGCTCGTTCCTGCGACAGGATCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACCGCCGAAATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCACAGACCATTGCGCGTTTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGGCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACCCTGCCTGATAACGCATTGCTGGCCGAAGGATTC
ACGCCTGCCCAGCTTGCCGCAGGGATAGAGCTGTATGAGGCGGTTGGCTGCGATGAGTGCACCGAAGGCTACAAGGGGCG
TACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGGAAGGCGGCAATGCAATGCAGATCG
CCGAAGCCGCGCAGGCGATCGGTATCCGCGATTTGCGTCAGTCGGCGCTGATGAAGGCTGCGAATGGGGTGACCAGCCTG
GCAGAGATCAACCGGGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.69

99.824

0.566
  pilB Acinetobacter baylyi ADP1

55.458

99.824

0.554
  pilB Legionella pneumophila strain ERS1305867

53.076

100

0.531
  pilB Vibrio cholerae strain A1552

48.866

100

0.492
  pilF Neisseria gonorrhoeae MS11

49.296

99.824

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

98.418

0.462
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

92.97

0.366
  pilF Thermus thermophilus HB27

39.423

91.388

0.36

Multiple sequence alignment