Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FG175_RS22010 Genome accession   NZ_CP041134
Coordinates   4559966..4560988 (-) Length   340 a.a.
NCBI ID   WP_141960260.1    Uniprot ID   -
Organism   Serratia marcescens strain WVU-010     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4554966..4565988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FG175_RS21980 (FG175_21980) - 4555767..4556279 (+) 513 WP_141960256.1 SprT family zinc-dependent metalloprotease -
  FG175_RS21985 (FG175_21985) endA 4556381..4557076 (+) 696 WP_038879275.1 deoxyribonuclease I -
  FG175_RS21990 (FG175_21990) rsmE 4557146..4557877 (+) 732 WP_060430995.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FG175_RS21995 (FG175_21995) gshB 4557888..4558838 (+) 951 WP_141960258.1 glutathione synthase -
  FG175_RS22000 (FG175_22000) - 4558984..4559547 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  FG175_RS22005 (FG175_22005) ruvX 4559547..4559969 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  FG175_RS22010 (FG175_22010) pilT 4559966..4560988 (-) 1023 WP_141960260.1 type IV pilus twitching motility protein PilT Machinery gene
  FG175_RS22015 (FG175_22015) - 4561009..4561716 (+) 708 WP_102985233.1 YggS family pyridoxal phosphate-dependent enzyme -
  FG175_RS22020 (FG175_22020) proC 4561736..4562557 (+) 822 WP_087762574.1 pyrroline-5-carboxylate reductase -
  FG175_RS22025 (FG175_22025) - 4562589..4563143 (+) 555 WP_019456092.1 YggT family protein -
  FG175_RS22030 (FG175_22030) yggU 4563140..4563430 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  FG175_RS22035 (FG175_22035) - 4563448..4564041 (+) 594 WP_060422645.1 XTP/dITP diphosphatase -
  FG175_RS22040 (FG175_22040) hemW 4564034..4565176 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  FG175_RS22045 (FG175_22045) - 4565214..4565648 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36446.80 Da        Isoelectric Point: 7.2889

>NTDB_id=369629 FG175_RS22010 WP_141960260.1 4559966..4560988(-) (pilT) [Serratia marcescens strain WVU-010]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLSAQQRESLRRLGQLDLALHRPGGE
RLRANVFQQRAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDTALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=369629 FG175_RS22010 WP_141960260.1 4559966..4560988(-) (pilT) [Serratia marcescens strain WVU-010]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAGTGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGCTCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAACGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCACTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATTGACGAGATCAACCGGCACCAGCCGCGGCACATTTTAACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGTCGGCGATCGCTGATTCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TACGGCGTTGCGCGCTGCGTTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTCGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

49.702

98.824

0.491
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.788

97.059

0.474
  pilT Legionella pneumophila strain ERS1305867

48.64

97.353

0.474
  pilT Legionella pneumophila strain Lp02

48.64

97.353

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Vibrio cholerae strain A1552

40.432

95.294

0.385
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment